Overview
- Unparalleled diversity of siRNA delivery and imaging strategies covered in a single volume
- Details the strategies that predict the design of effective siRNA with minimum side effects
- Fully explains miRNA biogenesis and how to design microRNA-based vectors and to apply them in vivo
- Explores the clinical potential of siRNA in human diseases, and describes the first application in patients with cancer
Part of the book series: Methods in Molecular Biology (MIMB, volume 487)
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About this book
RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection.
Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.
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Keywords
Table of contents (22 protocols)
Editors and Affiliations
Bibliographic Information
Book Title: siRNA and miRNA Gene Silencing
Book Subtitle: From Bench to Bedside
Editors: Mouldy Sioud
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-547-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-546-0Published: 26 November 2008
Softcover ISBN: 978-1-61737-908-6Published: 26 May 2011
eBook ISBN: 978-1-60327-547-7Published: 03 July 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 472
Topics: Biochemistry, general, Human Genetics, Gene Expression