Overview
- Provides neutrophil protocols that are broadly applicable to leukocyte biology
- Includes protocols for isolation of neutrophils from humans and animal species
- Presents an interesting and informative overview of the field of neutrophil biology
- Facilitates a better understanding of the role of neutrophils in host defense
Part of the book series: Methods in Molecular Biology (MIMB, volume 412)
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About this book
Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.
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Keywords
Table of contents (32 protocols)
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Neutrophils: An Overview
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Neutrophil Isolation and Subcellular Fractionation
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Biochemistry, Biology, and Signal Transduction of Neutrophils
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Neutrophil Adhesion and Chemotaxis
Reviews
From the reviews:
"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars
"This excellent book is one of the latest in the ‘Methods in Molecular Biology’ series … . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils – whether in research or diagnostics – to purchase one immediately. … I was impressed by this book’s comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)
Editors and Affiliations
Bibliographic Information
Book Title: Neutrophil Methods and Protocols
Editors: Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-467-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-788-4Published: 02 August 2007
Softcover ISBN: 978-1-61737-779-2Published: 10 December 2010
eBook ISBN: 978-1-59745-467-4Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XX, 537
Number of Illustrations: 104 b/w illustrations, 8 illustrations in colour
Topics: Immunology, Cell Biology, Human Genetics