Overview
- Highlights techniques involving a wider variety of cell types and organisms
- Contains state-of-the-art methods in step-by-step laboratory format
Part of the book series: Methods in Molecular Biology (MIMB, volume 435)
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About this book
Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.
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Keywords
Table of contents (16 protocols)
Reviews
From the reviews:
"I looked at Chromosomal Mutagenesis from the perspective of a geneticist … . it serves as a potentially useful text. … well written and protocols appear very detailed with good explanation that goes well beyond what one can glean from most published papers. … On the whole, for anyone either thinking of moving into a field where chromosomal mutagenesis is not so straightforward, or looking for ideas by learning how others have solved related problems, this book could serve as a very useful resource." (Alistair Goldman, Microbiology Today, May, 2008)
Editors and Affiliations
Bibliographic Information
Book Title: Chromosomal Mutagenesis
Editors: Gregory D. Davis, Kevin J. Kayser
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-232-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-899-7Published: 11 October 2007
Softcover ISBN: 978-1-61737-833-1Published: 09 December 2010
eBook ISBN: 978-1-59745-232-8Published: 15 March 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 236
Number of Illustrations: 36 b/w illustrations, 3 illustrations in colour
Topics: Biochemistry, general, Human Genetics, Cell Biology, Molecular Medicine