Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2062)
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About this book
This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes and macromolecular interaction of networks, and establish critical structural-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Keywords
Table of contents (27 protocols)
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Introduction to the RNA Exosome in Biomedicine
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Analyzing Exosome Substrates
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Exosomes and Their Supramolecular Functional Interactions
Editors and Affiliations
About the editors
Bibliographic Information
Book Title: The Eukaryotic RNA Exosome
Book Subtitle: Methods and Protocols
Editors: John LaCava, Štěpánka Vaňáčová
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9822-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-4939-9821-0Published: 26 November 2019
Softcover ISBN: 978-1-4939-9824-1Published: 26 November 2020
eBook ISBN: 978-1-4939-9822-7Published: 25 November 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 517
Number of Illustrations: 27 b/w illustrations, 52 illustrations in colour
Topics: Human Genetics, Cell Biology