Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1436)
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About this book
This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.
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Keywords
Table of contents (22 protocols)
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Class I, II, and, IV Histone Deacetylases
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Class III Deacetylases
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Editors and Affiliations
Bibliographic Information
Book Title: Histone Deacetylases
Book Subtitle: Methods and Protocols
Editors: Sibaji Sarkar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3667-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3665-6Published: 01 June 2016
Softcover ISBN: 978-1-4939-8111-3Published: 26 May 2018
eBook ISBN: 978-1-4939-3667-0Published: 31 May 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 337
Number of Illustrations: 21 b/w illustrations, 31 illustrations in colour
Topics: Cell Biology