Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2738)
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About this book
This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular techniques, protocols for isolation, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.
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Keywords
Table of contents (24 protocols)
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Front Matter
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An Overview of the Diversity of Bacteriophages
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Front Matter
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Isolation of Bacteriophages
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Front Matter
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Enumeration of Bacteriophages
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Bacteriophages
Book Subtitle: Methods and Protocols
Editors: Ebenezer Tumban
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3549-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-3548-3Published: 16 November 2023
Softcover ISBN: 978-1-0716-3551-3Due: 29 November 2024
eBook ISBN: 978-1-0716-3549-0Published: 15 November 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 431
Number of Illustrations: 11 b/w illustrations, 36 illustrations in colour
Topics: Microbiology, Immunology, Immunology