Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2596)
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About this book
The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
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Keywords
Table of contents (31 protocols)
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Introduction
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Difference Gel Electrophoresis
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DIGE Applications
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Comparative DIGE Tissue Proteomics
Editors and Affiliations
Bibliographic Information
Book Title: Difference Gel Electrophoresis
Book Subtitle: Methods and Protocols
Editors: Kay Ohlendieck
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2831-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-2830-0Published: 16 November 2022
Softcover ISBN: 978-1-0716-2833-1Published: 16 November 2023
eBook ISBN: 978-1-0716-2831-7Published: 15 November 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 485
Number of Illustrations: 8 b/w illustrations, 74 illustrations in colour
Topics: Chemistry/Food Science, general