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Plant Mitochondria

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Olivier Van Aken

    1. Department of Biology, Lund University, Lund, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Allan G. Rasmusson

    1. Department of Biology, Lund University, Lund, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2363)

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About this book

This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come.

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

    • Huy Cuong Tran, Olivier Van Aken
    Pages 1-12

  3. Cell Type–Specific Isolation of Mitochondria in Arabidopsis

    • Clément Boussardon, Olivier Keech
    Pages 13-23

  4. Isolation of Plant Mitochondria Using Affinity Purification

    • Franziska Kuhnert, Andreas P. M. Weber
    Pages 25-37

  5. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient

    • Ian Max Møller, Allan G. Rasmusson
    Pages 39-50

  6. Integrity Assessment of Isolated Plant Mitochondria

    • Allan G. Rasmusson, Mengshu Hao, Ian Max Møller
    Pages 51-62

  7. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores

    • Brendan M. O’Leary, Glenda Guek Khim Oh, A. Harvey Millar
    Pages 63-75

  8. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin

    • Allan G. Rasmusson, Ian Max Møller, Susanne Widell
    Pages 77-84

  9. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring Mass Spectrometry (SRM-MS)

    • Xuyen Le, A. Harvey Millar, Chun Pong Lee
    Pages 85-100

  10. Complexome Profiling of Plant Mitochondrial Fractions

    • Lucie Schröder, Holger Eubel, Hans-Peter Braun
    Pages 101-110

  11. High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes

    • Lilian Vincis Pereira Sanglard, Catherine Colas des Francs-Small
    Pages 111-119

  12. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy

    • Anqiang Tang, Yimin Wang, Nicolas L. Taylor
    Pages 121-152

  13. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana

    • Cristina Ruberti
    Pages 153-163

  14. Assessment of Mitochondrial Protein Topology and Membrane Insertion

    • Kerstin Schäfer, Carina Engstler, Korbinian Dischinger, Chris Carrie
    Pages 165-181

  15. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis

    • Malgorzata Kwasniak-Owczarek, Artur Tomal, Hanna Janska
    Pages 183-197

  16. Markers for Mitochondrial ROS Status

    • Monika Ostaszewska-Bugajska, Anna Podgórska, Bożena Szal
    Pages 199-213

  17. Mass Spectrometry–Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling

    • Jonas Giese, Jürgen Eirich, Frederik Post, Markus Schwarzländer, Iris Finkemeier
    Pages 215-234

  18. Analysis of the Plant Mitochondrial Transcriptome

    • Kevin Baudry, Etienne Delannoy, Catherine Colas des Francs-Small
    Pages 235-262

  19. Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing Data

    • Mizuki Takenaka
    Pages 263-278

  20. Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana Mitochondria

    • Omar Saleh, Sarlita Dwiani, Julia Rott, Kristina Kühn
    Pages 279-300
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Editors and Affiliations

  • Department of Biology, Lund University, Lund, Sweden

    Olivier Van Aken, Allan G. Rasmusson

Bibliographic Information

  • Book Title: Plant Mitochondria

  • Book Subtitle: Methods and Protocols

  • Editors: Olivier Van Aken, Allan G. Rasmusson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1653-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1652-9Published: 21 September 2021

  • Softcover ISBN: 978-1-0716-1655-0Published: 25 September 2021

  • eBook ISBN: 978-1-0716-1653-6Published: 20 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 342

  • Number of Illustrations: 18 b/w illustrations, 49 illustrations in colour

  • Topics: Plant Physiology, Plant Sciences

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