Overview
- Editors:
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Roberto J. Botelho
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Department of Chemistry and Biology and the Graduate Program in Molecular Science, Ryerson University, Toronto, Canada
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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About this book
This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.
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Table of contents (17 protocols)
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- Noah Steinfeld, Sai Srinivas Panapakkam Giridharan, Emily J. Kauffman, Lois S. Weisman
Pages 1-17
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- Avishek Ghosh, Padinjat Raghu
Pages 19-37
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- Gaëtan Chicanne, Justine Bertrand-Michel, Julien Viaud, Karim Hnia, Jonathan Clark, Bernard Payrastre
Pages 39-53
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- Rachel C. Wills, Jonathan Pacheco, Gerald R. V. Hammond
Pages 55-72
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- Rebecca Cabral-Dias, Yasmin Awadeh, Roberto J. Botelho, Costin N. Antonescu
Pages 73-89
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- Fan Fan, Chen Ji, Xuelin Lou
Pages 91-104
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- Jonathan Pacheco, Rachel C. Wills, Gerald R. V. Hammond
Pages 105-120
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- Mo Chen, Hudson T. Horn, Tianmu Wen, Vincent L. Cryns, Richard A. Anderson
Pages 133-142
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- Calvin Yeager, Djoshkun Shengjuler, Simou Sun, Paul S. Cremer, Craig E. Cameron
Pages 143-156
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- Julian Bender, Carla Schmidt
Pages 157-175
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- Mélanie Mansat, Mélanie Picot, Gaëtan Chicanne, Virginie Nahoum, Frédérique Gaits-Iacovoni, Bernard Payrastre et al.
Pages 177-184
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- Julien Viaud, Laurie Ceccato, Bernard Payrastre, Frédérique Gaits-Iacovoni
Pages 185-194
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- Yosuke Senju, Pekka Lappalainen, Hongxia Zhao
Pages 195-204
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- Ángel Pérez-Lara, Reinhard Jahn
Pages 205-214
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- Yosuke Senju, Hongxia Zhao
Pages 215-223
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- Adam J. Carr, Edhriz Siraliev-Perez, Weigang Huang, John Sondek, Qisheng Zhang
Pages 225-236
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Back Matter
Pages 237-239
Editors and Affiliations
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Department of Chemistry and Biology and the Graduate Program in Molecular Science, Ryerson University, Toronto, Canada
Roberto J. Botelho