Abstract
Formate dehydrogenases (FDHs) from different sources are systematically studied in our laboratory. Over the past few years, new genes of four FDHs from pathogenic bacterium Staphylococcus aureus (SauFDH), methylotrophic thermotolerant yeast Ogataea parapolymorpha (OpaFDH), yeast Saccharomyces cerevisiae (SceFDH), and moss Physcomitrella patens (PpaFDH) have been cloned and expressed in E. coli cells. By means of differential scanning calorimetry, a comparative study of thermal stability of new recombinant formate dehydrogenases and a number of FDHs from other sources has been performed. It was shown that two new enzymes, SauFDH and OpaFDH, are comparable to the FDH from Pseudomonas sp. 101 bacteria in their stability. SceFDH is the least stable FDH among the described formate dehydrogenases.
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Abbreviations
- DSC:
-
differential scanning calorimetry
- SauFDH, PseFDH, OpaFDH, CboFDH, SceFDH, AthFDH, SoyFDH, and PpaFDH:
-
recombinant formate dehydrogenases extracted from bacteria Staphylococcus aureus and Pseudomonas sp. 101, methylotrophic yeasts Ogataea parapolymorpha and Candida boidinii, baker’s yeast, and plants A. thaliana, Glycine max soya, and Physcomitrella patens, respectively
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Original Russian Text © A.A. Pometun, S.Yu. Kleymenov, S.A. Zarubina, I.S. Kargov, P.D. Parshin, E.G. Sadykhov, S.S. Savin, V.I. Tishkov, 2018, published in Vestnik Moskovskogo Universiteta, Seriya 2: Khimiya, 2018, No. 2, pp. 164–169.
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Pometun, A.A., Kleymenov, S.Y., Zarubina, S.A. et al. Comparison of Thermal Stability of New Formate Dehydrogenases by Differential Scanning Calorimetry. Moscow Univ. Chem. Bull. 73, 80–84 (2018). https://doi.org/10.3103/S002713141802013X
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DOI: https://doi.org/10.3103/S002713141802013X