Abstract
The excitation and emission spectra of dyes that could be used for indicator studies in the process of oilfield development (uranine, eosin Y, rhodamine B, rhodamine 6G, amino-G-acid) are investigated using a Flyuorat-02-Panorama spectrofluorimeter at different scanning regimes and at different pH values. Optimal conditions are elucidated for simultaneous separate determination: aqueous solution with pH 9.18 (borate buffer solution), λex = 300 nm (5 dyes) or 490 nm (4 dyes) with fluorescence spectra recording (registration scanning regime with spectrum cutoff in the region 480–580 or 500–600 nm, respectively); λex = 300 nm and Δλ = 25 nm with spectra recording in the range 300–680 nm using a regime of synchronous scanning (4 dyes). An algorithm is suggested for processing of spectra recorded using different methods. Dye concentrations in the mixture are calculated using the Panorama Pro, Version 2.1.0 software supplied with the instrument. The possibility of simultaneous separate determination of 2–5 dyes in the mixture was demonstrated.
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Original Russian Text © E.M. Basova, V.M. Ivanov, O.C. Apendeeva, 2014, published in Vestnik Moskovskogo Universiteta. Khimiya, 2014, No. 5, pp. 276–290.
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Basova, E.M., Ivanov, V.M. & Apendeeva, O.C. Capability of Flyuorat-02-Panorama spectrofluorimeter to analyze fluorescent dye mixtures. Moscow Univ. Chem. Bull. 69, 214–224 (2014). https://doi.org/10.3103/S0027131414050022
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DOI: https://doi.org/10.3103/S0027131414050022