Abstract
Escherichia coli O157:H7 is well known enterohemorrhagic pathogen responsible for infections among animals including a man. The main source of this bacterium is cattle, that is mostly asymptomatic and through that E. coli O157:H7 can simple transfer to food products. Therefore, there is a need for rapid, sensitive and specific detection method. The present work is focused on its detection by a heptaplex polymerase chain reaction, which targets genes from known virulent regions of E. coli O157:H7. According to obtained results this approach is able to reach the detection sensitivity of 4 colony-forming units (CFU) from a culture and 6 and 8 CFU from milk and meat samples, respectively, independently of tested sample volume.
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Abbreviations
- CFU:
-
colony-forming units
- EHEC:
-
enterohemorrhagic E. coli
- LB:
-
Luria-Bertani broth
- PBS:
-
phosphate buffered saline
- PCR:
-
polymerase chain reaction
- SMAC:
-
sorbitol MacConkey agar
- Stx:
-
shiga-like toxin
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Vidová, B., Tóthová, E., Blahut, Ľ. et al. Multiplex PCR for detection of Escherichia coli O157:H7 in foods. Biologia 66, 401–405 (2011). https://doi.org/10.2478/s11756-011-0052-z
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DOI: https://doi.org/10.2478/s11756-011-0052-z