Abstract
The article presents research findings on two human strains with probiotic activity. On the basis of API 50 CHL fermentation pattern, PCR by species-specific primers and sequencing of the V2–V3 region of 16S rRNA both strains designated as LF221 and K7 were identified as members of the Lactobacillus gasseri species. Two LF221 bacteriocins, acidocin LF221 A and B were purified and sequenced. They were classified as members of the two-component class II bacteriocins. Among basic probiotic properties, the survival under conditions in gastro-intestinal tract, ability to adhere to cultured intestinal enterocytes and pig’s mucosa and stimulation of the immune response were demonstrated. In in vivo study of 24 weaned piglets, the survival rate of K7 Rifr and LF221 Rifr was quantified by selective enumeration on MRS agar with rifampicin. The survival of both strains was good (2.9 × 105 cfu of K7 Rifr /g faeces; 4.8 × 105 cfu of LF221 Rifr /g) and the LF221 Rifr /K7 Rifr viable cells were found either in the mucosa of duodenum, jejunum or in the ileum. The possible effect of K7 to inhibit adhesion of E. coli O8:K88 to enterocytes was studied on Caco-2 cultured cells, on tissue obtained from small intestines of pigs and in vivo on gnotobiotic piglets. Lactobacilli were found to be effective in reducing E. coli adhesion to enterocytes in Caco-2 model, but not on mucosa of pig’s jejunum under ex vivo conditions. Competitive exclusion, production of organic acids and stimulation of immune response, were involved in inhibition of E. coli by K7 strain in gnotobiotic piglets. Any inflammatory change in intestines of piglets treated with K7 was observed, which confirmed its safe use. Among the technological parameters the survival and activity of the strains during cheese-making are presented.
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Abbreviations
- GIT:
-
gastrointestinal tract
- LAB:
-
lactic acid bacteria
- RAPD:
-
random amplified polymorphic DNA
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Rogelj, I., Bogovič Matijašić, B. Lactobacillus gasseri LF221 and K7 — from isolation to application. Biologia 61, 761–769 (2006). https://doi.org/10.2478/s11756-006-0154-1
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DOI: https://doi.org/10.2478/s11756-006-0154-1