Summary
In the present study, a simple and efficient high-performance thin-layer chromatographic (HPTLC) method was developed for the separation and quantitation of three proton-pump inhibitors, omeprazole, pantoprazole, and rabeprazole, from their binary combinations with diclofenac. Using a “quality by design” approach, preliminary trials were performed on pre-coated silica gel HPTLC plates using toluene together with various alcohols (methanol, ethanol, iso-propanol, n-butanol) as the mobile phase. For better peak symmetry, ammonia was added in different volumes, and its effect on analyte retention and separation was also assessed. The mobile phase consisting of toluene-n-butanol-25% ammonia (3:7:0.2, v/v) afforded excellent separation of proton-pump inhibitors from diclofenac as well as from each other. The retardation factor (RF) for all the separated compounds was between 0.20 and 0.80. The developed method was successfully validated as per the International Conference on Harmonization (ICH) guidelines, and the selected drugs were determined simultaneously from dosage forms without any interference from the excipients.
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Bhatt, N.M., Chavada, V.D., Sanyal, M. et al. Optimization of a Novel High-Performance Thin-Layer Chromatographic Method for the Concurrent Estimation of Three Proton-Pump Inhibitors and Diclofenac in Their Binary Combinations. JPC-J Planar Chromat 28, 234–240 (2015). https://doi.org/10.1556/1006.2015.28.3.7
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DOI: https://doi.org/10.1556/1006.2015.28.3.7