Abstract
Antioxidants can reduce the occurrence of long-term damages, caused by free radicals. In this study, we aimed to evaluate the effects of CuO nanoparticles and hydroalcoholic extracts of Berberis vulgaris, Descurainia sophia and Silybum marianum on catalase, glutathione peroxidase, and malondialdehyde concentrations in male diabetic rats. In case 50 Wistar rats (250–350 g) were divided in ten groups (five rats per group): healthy controls, healthy rats receiving nanoparticles, healthy rats receiving B. vulgaris, D. sophia, and S. marianum extracts (independently), diabetic controls, diabetic rats receiving CuO nanoparticles, and diabetic rats receiving the extracts independently. In diabetic groups, diabetes was induced in half of the rats, using alloxan at a dose of 120 mg kg−1. In addition to CuO nanoparticles, the control and diabetic groups independently received 0.5 ml of B. vulgaris, D. sophia, and S. marianum extracts via intraperitoneal injection for 30 days. Then, the animals were anesthetized with ketamine and the liver tissues were removed. The concentrations of catalase, glutathione peroxidase, and malondialdehyde were measured and compared. In diabetic groups treated with CuO nanoparticles, a significant increase was reported in the concentration of malondialdehyde (from 4.7 ± 0.44 to 5.05 ± 0.40) (mean ± SD). Moreover, a significant decline was observed in the activity of catalase (from 36.8 ± 1.48 to 36.2 ± 1.48) and glutathione peroxidase enzymes (from 75.4 ± 3.91 to 72.4 ± 4.33). The results show that S. marianum extract was more effective than B. vulgaris and D. sophia extracts in diminishing the effects of CuO nanoparticles.
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Abbreviations
- CAT:
-
catalase
- MDA:
-
malondialdehyde
- GPx:
-
glutathione peroxidase
- SOD:
-
superoxide dismutase
- DTNB:
-
5,5’-dithiobis-(2-nitrobenzoic acid)
- XRD:
-
X-ray diffraction
- TEM:
-
transmission electron microscope
- SEM:
-
scanning electron microscope
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Mohammadifard, M., Mottaghipisheh, J. The effects of ethanolic herbal extracts and CuO nanoparticles on catalase, glutathione peroxidase and malondialdehyde in male diabetic rats. Biologia 72, 357–363 (2017). https://doi.org/10.1515/biolog-2017-0031
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DOI: https://doi.org/10.1515/biolog-2017-0031