Abstract
The aim of the present study was to compare structure of the nucleoli of ependymocytes, tanycytes, and secretory cells of the subcommissural organ using immunohistochemical staining for nucleolin and confocal laser microscopy. The study was performed in samples from the diencephalon of adult male Wistar rats (n = 6). The samples were fixed in zinc–ethanol–formaldehyde, a fixative providing a high level of preservation of antigen determinants. In the present study, we estimated diameters of nucleoli and their number in various types of cells lining the third ventricle. We compared for the first time the nucleoli of different subpopulations of tanycytes and report data on the distribution of nucleolin protein in the cells lining the ventricles. The content and location of nucleolin reflect the functional state of the cell. Our data will promote understanding of the interrelationships between the indices of the nucleolar apparatus and the functional state of the cell under various conditions, including stress, neoplastic transformation, and other pathological conditions.
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Abbreviations
- SCO:
-
subcommissural organ
- CSF:
-
cerebrospinal fluid
- CVO:
-
circumventricular organs
References
Chen, Z. and Xu, X., Roles of nucleolin, focus on cancer and anti-cancer therapy, Saudi Med., 2016, vol. J 37, pp. 1312–1318.
Del Bigio, M.R., Ependymal cells: biology and pathology, Acta Neuropathol., 2010, vol. 119, pp. 55–73.
Derenzini M., Brighenti, E., Donati, G., Vici, M., Ceccarelli, C., Santini, D., Taffurelli, M., Montanaro, L., and Treré, D., The P53-mediated sensitivity of cancer cells to chemotherapeutic agents is conditioned by the status of the retinoblastoma protein, J. Pathol., 2009, vol. 219, pp. 373–382.
Farley, K.I., Surovtseva, Y., Merkel, J., and Baserga, S.J., Determinants of mammalian nucleolar architecture, Chromosoma, 2015, vol. 124, pp. 323–331.
Goodman, T. and Hajihosseini, M.K., Hypothalamic tanycytes— masters and servants of metabolic, neuroendocrine, and neurogenic functions, Front. Neurosci., 2015, vol. 9, pp. 2–9.
Grondona, J.M., Hoyo-Becerra, C., Visser, R., Fernández-Llebrez, P., and López-Ávalos, M.D., The subcommissural organ and the development of the posterior commissure, Int. Rev. Cell Mol. Biol., 2012, vol. 296, pp. 63–137.
Guerra, M.M., González, C., Caprile, T., Jara, M., Vío, K, Muñoz, R.I., Rodríguez, S., and Rodríguez, E.M., Understanding how the subcommissural organ and other periventricular secretory structures contribute via the cerebrospinal fluid to neurogenesis, Front. Cell. Neurosci., 2015, vol. 9, pp. 1–17.
Hetman, M. and Pietrzak, M., Emerging roles of the neuronal nucleolus, Trends Neurosci., 2012, vol. 35, pp. 305–314.
Holmberg Olausson, K., Elsir, T., Moazemi Goudarzi, K., Nistér, M., and Lindström, M.S., NPM1 histone chaperone is upregulated in glioblastoma to promote cell survival and maintain nucleolar shape, Sci. Rep., 2015, vol. 5, pp. 1–15.
Joly, J.S., Osório, J., Alunni, A., Auger, H., Kano, S., and Rétaux, S., Windows of the brain: towards a developmental biology of circumventricular and other neurohemal organs, Semin. Cell Dev. Biol., 2007, vol. 18, pp. 512–524.
Kaur, C. and Ling, E.A., The circumventricular organs, Histol. Histopathol., 2017, vol. 32, pp. 879–892.
Kirik, O.V. and Korzhevskii, D.E., Vimentin in ependymal cells and subventricular proliferative zone cells of rat telencephalon, Bull. Exp. Biol. Med., 2013, vol. 154, pp. 553–557.
Korzhevskii, D.E., Choroid plexus and structural organization of blood-CSF barrier in human, Reg. Krovoobr. Mikrotsirk., 2003, vol. 2, no. 1, pp. 5–14.
Lafarga, M., Berciano, M.T., Saurez, I., Andres, M.A., and Berciano, J., Reactive astroglia–neuron relationships in the human cerebellar cortex: a quantitative, morphological and immunocytochemical study in Creutzfeldt–Jakob disease, Int. J. Dev. Neurosci., 1993, vol. 11, pp. 199–213.
Langlet, F., Mullier, A., Bouret, S.G., Prevot, V., and Dehouck, B., Tanycyte-like cells form a blood–cerebrospinal fluid barrier in the circumventricular organs of the mouse brain, J. Comp. Neurol., 2013, vol. 521, pp. 3389–3405.
Miranda, E., Almonacid, J.A., Rodriguez, S., Perez, J., Hein, S., Cifuentes, M., Fernández-Llebrez, P., and Rodríguez, E.M., Searching for specific binding sites of the secretory glycoproteins of the subcommissural organ, Microsc. Res. Tech., 2001, vol. 52, pp. 541–551.
Németh, A. and Längst, G., Chromatin organization and the mammalian nucleolus, in: Proteins of the Nucleolus. Regulation, Translocation, and Biomedical Functions, Netherlands: Springer, 2013, pp. 119–148.
Nurnberger, F. and Schoniger, S., Presence and functional significance of neuropeptide and neurotransmitter receptors in subcommissural organ cells, Microsc. Res. Tech., 2001, vol. 52, pp. 534–540.
Parlato, R. and Kreiner, G., Nucleolar activity in neurodegenerative diseases: a missing piece of the puzzle? J. Mol. Med. (Berlin), 2013, vol. 91, pp. 541–547.
Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates, 6th ed., Elsevier Inc., 2007.
Pena, E., Berciano, M.T., Fernandez, R., Ojeda, J.L., and Lafarga, M., Neuronal body size correlates with the number of nucleoli and cajal bodies, and with the organization of the splicing machinery in rat trigeminal ganglion neurons, J. Comp. Neurol., 2001, vol. 430, pp. 250–263.
Rodríguez, E.M., Blázquez, J.L., and Guerra, M., The design of barriers in the hypothalamus allows the median eminence and the arcuate nucleus to enjoy private milieus: the former opens to the portal blood and the latter to the cerebrospinal fluid, Peptides, 2010, vol. 31, pp. 757–776.
Sufieva, D.A., Kirik, O.V., Alekseeva, O.S., and Korzhevskii, D.E., Intermediate filament proteins in tanycytes of the third cerebral ventricle in rats during postnatal ontogenesis, J. Evol. Biochem. Physiol., 2016, vol. 52, no. 6, pp. 490–498.
Tajrishi, M.M., Tuteja, R., and Tuteja, N., Nucleolin, the most abundant multifunctional phosphoprotein of nucleolus, Commun. Integr. Biol., 2011, vol. 4, pp. 267–275.
Treré, D., Ceccarelli, C., Montanaro, L., Tosti, E., and Derenzini, M., Nucleolar size and activity are related to pRb and p53 status in human breast cancer, J. Histochem. Cytochem., 2004, vol. 52, pp. 1601–1607.
Wallace, H., Nucleolar growth and fusion during cellular differentiation, J. Morphol., 1963, vol. 112, pp. 261–278.
Watanabe-Susaki, K., Takada, H., Enomoto, K., Miwata, K., Ishimine, H., Intoh, A., Ohtaka, M., Nakanishi, M., Sugino, H., Asashima, M., and Kurisaki, A., Biosynthesis of ribosomal RNA in nucleoli regulates pluripotency and differentiation ability of pluripotent stem cells, Stem Cells, 2014, vol. 32, pp. 3099–3111.
Zenit-Zhuravleva, E.G., Polkovnichenko, E.M., Lushnikova, A.A., Treshchalina, E.M., Bukaeva, I.A., and Raikhlin, N.T., Nucleophosmin and nucleolin: encoding genes and expression in various human and animal tissues, Mol. Med., 2012, vol. 4, pp. 24–31.
Zharskaya, O.O. and Zatsepina, O.V., Dynamics and mechanisms of the nucleolus reorganization during mitosis, Tsitologiia, 2007, vol. 49, no. 5, pp. 355–369.
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Original Russian Text © D.A. Sufieva, O.V. Kirik, D.E. Korzhevskii, 2018, published in Tsitologiya, 2018, Vol. 60, No. 1, pp. 21–29.
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Sufieva, D.A., Kirik, O.V. & Korzhevskii, D.E. Nucleolin and Nucleoli in Ependymocytes and Tanycytes of the Third Ventricle of the Rat Brain. Cell Tiss. Biol. 12, 167–173 (2018). https://doi.org/10.1134/S1990519X18020116
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DOI: https://doi.org/10.1134/S1990519X18020116