Abstract
We carried out an in vitro study to examine the effects of testosterone and β-estradiol on activation of naive (CD45RA+) and primed (CD45RO+) T cells based on the production of IL-2 and expression of the CD25 (IL-2Rα) molecule. We determined that, overall, testosterone exerts an inhibitory effect on the functional activity of both naive and primed T cells. Naive cells were more sensitive to the testosterone effects, than primed lymphocytes. We identified a dose-dependent suppressive effect of β-estradiol on production of IL-2 by the activated CD45RA+ and CD45RO+ lymphocytes in the course of a uniform decrease in the number of CD25-positive T cells.
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Abbreviations
- mAb:
-
monoclonal antibodies
- Ac/Exp:
-
Human T Cell Activation/Expansion Kit (activator of T lymphocytes)
- CD:
-
cluster of differentiation
- Est:
-
estradiol
- IL:
-
interleukin
- Test:
-
testosterone
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Original Russian Text © A.A. Gutsol, N.A. Sokhonevich, K.A. Kofanova, L.S. Litvinova, 2014, published in Tsitologiya, 2014, Vol. 56, No. 7, pp. 500–503.
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Gutsol, A.A., Sokhonevich, N.A., Kofanova, K.A. et al. The effects of testosterone and β-estradiol on activation of lymphocytes associated with IL-2 production and expression of CD25 (IL-2Rα). Cell Tiss. Biol. 8, 473–477 (2014). https://doi.org/10.1134/S1990519X14060030
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DOI: https://doi.org/10.1134/S1990519X14060030