Abstract
The dynamics of the oxidative metabolism of the blood of healthy donors (n = 30) who were exposed in vitro to nitric oxide in the form of gas or dinitrosyl iron complexes has been investigated. The intensity of lipid peroxidation and the level of malonic dialdehyde in plasma and red blood cells were determined, as well as the plasma antioxidant capacity and the level of superoxide dismutase activity in the blood samples. This study is the first to have characterized the specific features of the response of proand antioxidant systems of the blood to free or bound (into dinitrosyl iron complexes) nitrogen monoxide in vitro; in particular, the gas flow from the Plazon device was shown to have a pronounced prooxidant effect, which was moderately alleviated by tenfold dilution of the NO-containing mixture. The use of an experimental nitric oxide generator that was constructed at the Russian Federal Nuclear Center resulted in minimal pro-oxidant activity, while an aqueous solution of dinitrosyl iron complexes had a moderate antioxidant effect that was manifested as the limitation of lipid peroxidation in blood plasma and an increase of superoxide dismutase activity in erythrocytes.
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Abbreviations
- DNIC:
-
dinitrosyl iron complexes
- MDA:
-
malonic dialdehyde
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Original Russian Text © A.K. Martusevich, A.G. Soloveva, S.P. Peretyagin, A.F. Vanin, 2015, published in Biofizika, 2015, Vol. 60, No. 2, pp. 348–354.
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Martusevich, A.K., Soloveva, A.G., Peretyagin, S.P. et al. A comparative analysis of the effects of free and bound NO on Pro- and antioxidant systems of the blood. BIOPHYSICS 60, 278–283 (2015). https://doi.org/10.1134/S0006350915020104
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DOI: https://doi.org/10.1134/S0006350915020104