Abstract
It has been shown that a producer strain of reactivating factor (RF) is identical to a typical strain of Luteococcus japonicus DSM 10546 from the Propionibacteriaceae family according to the physiological and biochemical properties and the sequencing of 16S rRNA fragments. A number of phenotypical differences from the model strain allowed the producer strain to be considered a subspecies of Luteococcus japonicus, and it was named Luteococcus japonicus subsp. casei. At cultivation of the producer, RF is secreted into the medium and plays the role of a signaling molecule. RF antioxidant activities towards various organic radicals may be a possible mechanism of its protective and reactivating effects.
Metabolites secreted by the L. casei producer strain into the culture medium were separated by a combination of liquid chromatographies. Four components possessing biological activities were found. The most active one was studied by MALDI-TOF mass spectrometry, which revealed that it is a polypeptide. Primary identification of some amino acid residues was performed. Sugar residues were found in the structure.
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Szent-Gyorgyi, A., Bioelektronika (Bioelectronics), Moscow: Mir, 1971.
Golod, N.A., Loiko, N.G., Lobanov, K.V., Mironov, A.S., Voeikova, T.A., Gal’chenko, V.F., Nikolaev, Yu.A., and El’-Registan, G.I., Microbiology (Moscow), 2009, vol. 78, no. 6, pp. 678–688.
El’-Registan, G.I., Mulyukin, A.L., Nikolaev, Yu.A., Suzina, N.E., Gal’chenko, V.F., and Duda, V.I., Microbiology (Moscow), 2006, vol. 75, no. 4, pp. 380–389.
Goh, E.B.YimG., Tsui, W., Meclure, J., Surette, M.G., and Davies, J., Proc. Natl. Acad. Sci. USA, 2002, vol. 99, no. 26, pp. 17025–17030.
Vorob’eva, L.I., Khodzhaev, E.Yu., Novikova, T.M., Mulyukin, A.L., Chudinova, E.M., Kozlova, A.N., and El’-Registan, G.I., Microbiology (Moscow), 2013, vol. 82, no. 5, pp. 594–599.
Rowbury, R.J., Sci. Prog., 2003, vol. 86, nos. 1/2, pp. 139–156.
Rowbury, R.J., Sci. Prog., 2003, vol. 86, no. 4, pp. 313–332.
Rowbury, R.J. and Goodson, M., Sci. Prog., 2001, vol. 84, no. 3, pp. 205–233.
Vorob’eva, L.I., Khodzhaev, E.Yu., and Ponomareva, G.M., Appl. Biochem. Microbiol., 2008, vol. 44, no. 1, pp. 38–41.
Vorob’eva, L.I., Khodzhaev, E.Yu., Mulyukin, A.L., and Toropygin, I.Yu., Appl. Biochem. Microbiol., 2009, vol. 45, no. 5, pp. 489–493.
Bulygina, E.S., Kuznetsov, V.V., Marusina, A.I., Turova, T.P., Kravchenko, I.K., Bykova, S.A., Kolganova, T.V., and Gal’chenko, V.F., Microbiology (Moscow), 2002, vol. 71, no. 4, pp. 425–432.
Edwards, U., Rogall, T., Bloeker, H., Ende, M.D., and Boeettge, E.C., Nucleic Acids Res., 1989, vol. 17, no. 19, pp. 7843–7853.
Thompson, J.D., Higgins, D.G., and Gibson, T.J., Nucleic Acids Res., 1994, vol. 22, no. 22, pp. 4673–4680.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C., Free Radic. Biol. Med., 1999, vol. 26, nos. 9/10, pp. 1231–1237.
Ou, B., Hampsch-Woodill, M., and Prior, R.L., J. Agric. Food Chem., 2001, vol. 49, no. 10, pp. 4619–4626.
Moore, J., Cheng, Z., Su, L., and Yu, L., J. Agric. Food Chem., 2006, vol. 54, no. 24, pp. 9032–9045.
Alekseeva, A.A. and Vorob’eva, L.I., Chan Thi Thanh, Baranova N.A., Aleksandrushkina N.I, Mikrobiologiya, 1973, vol. 42, no. 3, pp. 464–467.
Vorob’eva, L.I., Turova, T.P., Kraeva, N.I., and Alekseeva, A.A., Mikrobiologiya, 1983, vol. 52, no. 3, pp. 465–470.
Stackenbrandt, E. and Ebers, J., Microbiol. Today, 2006, no. 1, pp. 152–155.
Vorob’eva, L.I., Khasaeva, F.M., Vasilyuk, N.V., and Treinkul, E., Microbiology (Moscow), 2011, vol. 80, no. 5, pp. 664–671.
Takeuchi, M. and Yokota, A., Int. J. Syst. Bacteriol., 1994, vol. 44, no. 2, pp. 348–356.
Vorob’eva, L.I., Khodzhaev, E.Yu., and Ponomareva, G.M., Microbiology (Moscow), 2003, vol. 72, no. 4, pp. 428–433.
Kusano, K., Yamada, H., Niwa, M., and Yamasato, K., Int. J. Syst. Bacteriol., 1997, vol. 47, no. 4, pp. 825–831.
Collins, M.D., Lawson, P.A., Nikolaitchouk, N., and Falsen, E., Int. J. Syst. Evol. Microbiol., 2000, vol. 50, no. 1, pp. 179–181.
Collins, M.D., Hutson, R.A., Nikolaitchouk, N., and Falsen, E., Int. J. Evol. Microbiol., 2003, vol. 53, no. 6, pp. 1889–1891.
Vorobjeva, L.I. and Kraeva, N.I., Arch. Microbiol., 1982, vol. 113, no. 2, pp. D.110–113.
Vorob’eva, L.I., Al’-Sudani, S., and Kraeva, N.I., Mikrobiologiya, 1986, vol. 55, no. 5, pp. 750–753.
Vorobjeva, L.I., Khodzhaev, E.Yu., Kharchenko, N.V., Novikova, T.M., and Cherdyntseva, T.A., Appl. Biochem. Microbiol., 2014, vol. 50, no. 4, pp. 346–352.
Prior, R.L., Wu, X., and Schaich, K., J. Agric. Food Chem., 2005, vol. 53, no. 10, pp. 4290–4302.
Pazos, M., Andersen, M.L., and Skibsted, L.H., J. Agric. Food Chem., 2006, vol. 54, no. 26, pp. 10215–10221.
Tsopmo, A., Food Chem., 2011, vol. 126, no. 3, pp. 1138–1143.
Hernandez-Ledesma, B., Miralles, B., Amigo, L., Ramos, M., and Recio, I., J. Sci. Food Agric., 2005, vol. 85, no. 6, pp. 1041–1048.
Clausen, M.R., Skibsted, L.H., and Stagsted, J., J. Agric. Food Chem., 2009, vol. 57, no. 7, pp. 2912–2919.
Gonzales-Rodriges, I., Ruis, L., Guemonde, M., Abelardo, M., and Sanches, B., FEMS Microbiol. Letts., 2013, vol. 340, no. 1, pp. 1–10.
Dziuba, M. and Darewicz, M., Food Sci. Technol. Int., 2007, vol. 13, no. 6, pp. 393–404.
Sarmadi, B.H. and Ismail, A., Peptides, 2010, vol. 31, no. 10, pp. 1949–1956.
Samokhina, L.S., Komolova, G.S., Ganina, V.I., Ionova, I.I., and Semenov, G.V., Izv. Vuzov. Pishch. Tekhnol., 2012, nos. 5–6, pp. 17–20.
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Original Russian Text © L.I. Vorob’eva, E.A. Rogozhin, E.Yu. Khodzhaev, I.V. Nikolaev, T.P. Turova, 2015, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2015, Vol. 51, No. 1, pp. 37–45.
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Vorob’eva, L.I., Rogozhin, E.A., Khodzhaev, E.Y. et al. Reactivating factor of Luteococcus japonicus subsp. casei: Isolation and characterization. Appl Biochem Microbiol 51, 44–51 (2015). https://doi.org/10.1134/S0003683814060167
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DOI: https://doi.org/10.1134/S0003683814060167