Abstract
Methylosinus trichosporium OB3b oxidized methane to methanol in the presence of a high concentration of Cu2+. Further oxidation of methanol to formaldehyde was prevented by adding 200 mm NaCl which acted as a methanol dehydrogenase H inhibitor. The bacterium, 0.6 mg dry cell ml−1, in methane/air (1:4, v/v) at 25 °C in 12.9 mm phosphate buffer (pH 7) containing 20 mm sodium formate and 200 mm NaCl accumulated 7.7 mm methanol over 36 h.
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Lee, S.G., Goo, J.H., Kim, H.G. et al. Optimization of methanol biosynthesis from methane using Methylosinus trichosporium OB3b. Biotechnology Letters 26, 947–950 (2004). https://doi.org/10.1023/B:bile.0000025908.19252.63
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DOI: https://doi.org/10.1023/B:bile.0000025908.19252.63