Abstract
Rhodobacter sphaeroides AS1.1737 decolorized more than 90% of several azo dyes (200 mg dyes l−1) in 24 h. The optimal culture conditions were: anaerobic illumination (1990 lx), peptone as carbon source, temperature 35–40 °C and pH 7–8. Intracellular crude enzyme from this strain had azoreductase activity, optimized temperature as 45–50 °C, and decolorization kinetics which were consistent with a ping-pong mechanism.
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Chen BY (2002) Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola: toxicity and kinetics. Proc. Biochem. 38: 437–446.
Nakanishi M, Yatome C, Ishida N, Kitade Y (2001) Putative ACP phosphodiesterase gene (acpD) encodes an azoreductase. J. Biol. Chem. 276: 46394–46399.
O'Neill C, Lopez A, Esteves S, Hawkes FR, Hawkes DL, Wilcox S (2000) Azo-dye degradation in an anaerobic-aerobic treatment system operating on simulated textile effluent. Appl. Microbiol. Biotechnol. 53: 249–254.
Sponza DT, Isik M (2002) Decolorization and azo dye degradation by anaerobic/aerobic sequential process. Enzyme Microb. Technol. 31: 102–110.
Suzuki Y, Yoda T, Ruhul A, Sugiura W (2001) Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J. Biol. Chem. 276: 9059–9065.
Tan NCG, Prenafeta-Boldú FX, Opsteeg JL, Lettinga G, Field JA (1999) Biodegradation of azo dyes in cocultures of anaerobic granular sludge with aerobic aromatic amine degrading enrichment cultures. Appl. Microbiol. Biotechnol. 51: 865–871.
Yang QX, Yang M, Pritsch K, Yediler A, Hagn A, Schloter M, Kettrup A (2003) Decolorization of synthetic dyes and production of manganese-dependent peroxidase by new fungal isolates. Biotechnol. Lett. 25: 709–713.
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Song, Zy., Zhou, Jt., Wang, J. et al. Decolorization of azo dyes by Rhodobacter sphaeroides . Biotechnology Letters 25, 1815–1818 (2003). https://doi.org/10.1023/A:1026244909758
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DOI: https://doi.org/10.1023/A:1026244909758