Abstract
We determined the complete nucleotide sequence of the YL strain of Japanese encephalitis virus and its amino acid sequence was deduced. Our results displayed that the genome of YL strain contained a single open reading frame of 10,296 nucleotides (nts) which was flanked by untranslated region (UTR) containing 95 bases at the 5′-end and 586 bases at the 3′-end, respectively. Comparison of sequences showed that the overall amino acid sequence and 3′ UTR of YL were similar to those of the virulent strain JaGAr01. However, some significant amino acid differences of viral envelope (E) protein were observed between YL and JaGAr01; the amino acid sequence of E protein in YL strain possessed RGG(387–389) tripeptide instead of RGD(387–389) in JaGAr01 and in other strains; and another amino acid is K(138) in YL, not E(138) found in others. These differences suggested that the YL strain impairs in viral attachment to the cell surface and loses neuroinvasiveness, and therefore this strain was used as a live attenuated vaccine.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Vaughn D.W. and Hoke C.H., Epidemiol Rev 14, 197-221, 1992.
Lobigs M., Usha R., Nestorowicz A., Marshall I.D., Weir R.C., and Dalgarno L., Virology 176, 587-595, 1990.
Mishin V.P., Cominelli F., Yamshchikov V.F., Virus Res 81, 113-123, 2001.
Su C.M., Liao C.L.H., Lee Y.L., and Lin Y.L., Virology 66, 5425-5431, 2000.
Zhang F., Huang Q., Ma W., Jiang S., Fan Y., and Zhang H., J Virol Meth 96, 171-182, 2001.
Chamber T.J., Hahn C.S., Galler R., and Rice C.M., Annu Rev Microbiol 44, 649-688, 1990.
McMinn P.C., J Gen Virol 78, 2711-2722, 1997.
Rice C.M., in Fields B.N., Knipe D.M., and Howley P.M. Fields Virology, 3rd edn. Lippincott-Raven, Philadelphia, 1996, pp. 931-959.
Batista W.C., Kashima S., Marques A.C., and Figueiredo L.T., Virus Res 75, 35-42, 2001.
Mangada M.N.M. and Takegami T., Virus Res 59, 101-112, 1999.
Tsarev S.A., Sanders M.L., Vaughn D.W., and Innis B.L., Vaccine 286, 206-215, 2000.
Wu S.C., Lian W.C., Hsu L.C., and Liau M.Y., Virus Res 51, 173-181, 1997.
Chen L.K., Lin Y.L., Liao C.L., Lin C.G., Huang Y.L., and Yeh C.T., Virology 223, 79-88, 1996.
Monath T.P., Arroyo J., Levenbook I., Zhang Z.X., Catalan J., Draper K., and Guirakhoo F., J Virol 76, 1932-1943, 2002.
Seif S.A., Kouichi M., Sachiko M., Futoshi H., and Akira I., Vaccine 13, 1515-1521, 1995.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, JJ., Tsai, TH., Chang, TJ. et al. Cloning and Sequencing of Complete cDNA of Japanese Encephalitis Virus YL Strain in Taiwan. Virus Genes 26, 193–198 (2003). https://doi.org/10.1023/A:1023443631659
Issue Date:
DOI: https://doi.org/10.1023/A:1023443631659