Abstract
Inoculum size (1.5-6.0g dry weight/l) significantly affected cell growth and accumulation of intracellular and extracellular taxol in Taxus chinensis. A shorter cultivation time and a higher biomass productivity were achieved using inoculum size of 6.0g DW/l. Both the intracellular content and total production of taxol were increased almost 30% with an increase of inoculum size from 1.5 to 3.0g DW/l, while an even higher inoculum size decreased taxol formation. The extracellular taxol concentration was relatively higher (0.091mg/l) at low inoculum sizes of 1.5 and 2.0g DW/l; and in various cases it was less than 25% of the total amount of taxol produced.
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Wang, HQ., Zhong, JJ. & Yu, JT. Enhanced production of taxol in suspension cultures of Taxus chinensis by controlling inoculum size. Biotechnology Letters 19, 353–356 (1997). https://doi.org/10.1023/A:1018355002041
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DOI: https://doi.org/10.1023/A:1018355002041