Abstract
A 4.5 litre photobioreactor was developed and used to produce 13 C-labelled b-carotene by feeding 13 CO2 to growing ells of the marine alga Dunaliella salina. Three runs were carried out using different feeding regimes producing b-carotene containing 40, 56 and 76 Atom% 13 C. Approximately 145 mg b-carotene was recovered from each run. Incorporation of 13 C into b-carotene ranged from 1.4 to 2.8%. The labelled b-carotene produced would allow multiple tracer experiments for the study of human carotenoid metabolism.
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Wilson, P., Hilton, M., Waspe, C. et al. Production of 13C-labelled β-carotenefrom Dunaliella salina. Biotechnology Letters 19, 401–405 (1997). https://doi.org/10.1023/A:1018323522514
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DOI: https://doi.org/10.1023/A:1018323522514