Abstract
Cell suspension cultures of Taxus chinensis, supplemented with 25 g sucrose l−1, produced 11 mg cephalomanine l−1, 21 g biomass l−1 and 19 nkat geranylgeranyl diphosphate (GGPP) synthase activity g protein−1. Supplementation of the cultures with 100 μM methyl jasmonate (MJA) produced 17 mg cephalomanine l−1, 6 g biomass l−1 and 78 nkat GGPP synthase activity g protein−1. Addition of sucrose and MJA together produced 24 mg cephalomanine l−1, 18 g biomass l−1 and 55 nkat GGPP synthase activity g protein−1.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ketchum REB, Gibson DM, Groteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol. Bioeng. 62: 97-105.
Kim JK, Yun JH, Hwang YS, Byun SY, Kim DI (1995) Production of taxol and related taxanes in Taxus brevifolia cell cultures: effect of sugar. Biotechnol. Lett. 17: 101-106.
Laskaris G, Jong CFD, Jaziri M, Van der Heijden R, Theodoridis G, Verpoorte R (1999a) Geranylgeranyl diphosphate synthase activity and taxane production in Taxus baccata cells. Phytochemistry 50: 939-946.
Laskaris G, Bounkhay M, Theodorodos G, Van der Heijden R, Verpoorte R, Jaziri M (1999b) Induction of geranylgeranyl diphosphate synthase activity and taxane accumulation in Taxus baccata cell cultures after elicitation by methyl jasmonate. Plant Sci. 147: 1-8.
Pandey RC, Yankov LK, Poulev A, Caccamese S (1998) Synthesis and separation of potential anticancer active dihalocephalomanine diastereomers from extraction of Taxus yunanensis. J. Nat. Prod. 61: 57-63.
Wang HQ, Yu JT, Zhong JJ (1999) Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy. Proc. Biochem. 35: 479-483.
Yukimune Y, Hara Y, Nomura E, Seto H, Yoshida S (2000) The configuration of methyl jasmonate affects paclitaxel and baccatin III production in Taxus cells. Phytochemistry 54: 13-17.
Zhang CH, Mei XG, Liu L, Yu LJ (2000) Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis. Biotechnol. Lett. 22: 1561-1564.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lan, W., Yu, L., Cheng, P. et al. Improvement of cephalomanine production in Taxus chinensis cells by a combination of sucrose and methyl jasmonate. Biotechnology Letters 24, 1253–1255 (2002). https://doi.org/10.1023/A:1016269627346
Issue Date:
DOI: https://doi.org/10.1023/A:1016269627346