Abstract
A flocculent strain of Saccharomyces cerevisiae S646-1B accumulated more Cu2+ (81 nmol mg−1 dry wt) than the isogenic (except for the marker genes ade1 and trp1 and the gene FLO1) non-flocculent strain S646-8D (30 nmol mg−1 dry wt), in the first 10 min of contact of the cells with Cu2+. Additionally, this strain flocculated in solutions of 0.2 mM Cu2+, Ni2+, Zn2+ and Cd2+. The potential of using flocculent strains in the bioremediation of heavy metals contaminated waste waters is discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Avery SV, Tobin JM(1992) Mechanisms of strontium uptake by laboratory and brewing strains of Saccharomyces cerevisiae. Appl. Environ. Microbiol. 58: 3883-3889.
Blackwell KJ, Tobin JM (1999) Cadmium accumulation and its effects on intracellular ion pools in a brewing strain of Saccharomyces cerevisiae. J. Ind. Microbiol. Biotechnol. 23: 204-208.
Blackwell KJ, Singleton I, Tobin JM (1995) Metal cation uptake by yeast: a review. Appl. Microbiol. Biotechnol. 43: 579-584.
Cassidy MB, Lee H, Trevors JT (1996) Environmental applications of immobilized microbial cells: a review. J. Ind. Microbiol. 16: 79-101.
Ferraz AI, Teixeira JA (1999) The use of flocculating brewer's yeast for Cr(III) and Pb(II) removal from residual wastewaters. Bioproc. Eng. 21: 431-437.
Gadd GM (1990) Fungi and yeasts for metal accumulation. In: Ehrlich HL, Brierley CL, eds. Microbial Mineral Recovery. New York: McGraw-Hill Inc, pp. 249-275.
Marques PA, Pinheiro HM, Teixeira JA, Rosa MF (1999) Removal efficiency of Cu2+, Cd2+ and Pb2+ by waste brewery biomass: pH and cation association effects. Desalination 124: 137-144.
Miki BLA, Poon NH, James AP, Seligy VL (1982) Possible mechanism for interactions governed by gene FLO1 in Saccharomyces cerevisiae. J. Bacteriol. 150: 878-889.
Simmons P, Singleton I (1996) A method to increase silver biosorption by an industrial strain of Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 45: 278-285
Soares EV, Seynaeve J (2000) Induction of flocculation of brewer's yeast strains of Saccharomyces cerevisiae by changing the calcium concentration and pH of culture medium. Biotechnol. Lett. 22: 1827-1832.
Soares EV, Duarte APRS, Soares HMVM (2000) Study of the suitability of 2-(N-morpholino) ethanesulfonic acid pH buffer for heavy metals accumulation studies using Saccharomyces cerevisiae. Chem. Spec. Bioavail. 12: 59-65.
Soares HMVM, Conde PCFL, Almeida AAN, Vasconcelos MTSD (1999a) Evaluation of N-substituted aminosulfonic acids pH buffers with a morpholinc ring for cadmium and lead speciation studies by electroanalytical techniques. Anal. Chim. Acta 394: 325-335.
Soares HMVM, Pinho SCP, Barros MGRTM (1999b) Influence of N-substituted aminosulfonic acids with a morpholinic ring pH buffers on the redox processes of copper or zinc ions: a contribution to speciation studies. Electroanalysis 11: 1312-1317.
Sousa MJ, Teixeira JA, Mota M (1992) Differences in the flocculation mechanism of Kluyveromyces marxianus and Saccharomyces cerevisiae. Biotechnol. Lett. 14: 213-218.
Stewart GG, Russell I (1981) Yeast flocculation. In: Pollock JRA, ed. Brewing Science, Vol. 2. London: Academic Press, pp. 61-92.
Stratford M (1992) Yeast flocculation: a new perspective. Adv. Microb. Physiol. 33: 1-72.
Teixeira JA, Mota M, Goma G (1990) Continuous ethanol production by a flocculating strain of Kluyveromyces marxianus: bioreactor performance. Bioproc. Eng. 5: 123-127.
Volesky B, Holan ZR (1995) Biosorption of heavy metals. Biotechnol. Prog. 11: 235-250.
Volesky B, May-Philips HA (1995) Biosorption of heavy metals by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 42: 797-806.
Volesky B, May H, Holan ZR (1993) Cadmium biosorption by Saccharomyces cerevisiae. Biotechnol Bioeng. 41: 826-829.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Soares, E.V., De Coninck, G., Duarte, F. et al. Use of Saccharomyces cerevisiae for Cu2+ removal from solution: the advantages of using a flocculent strain. Biotechnology Letters 24, 663–666 (2002). https://doi.org/10.1023/A:1015062925570
Issue Date:
DOI: https://doi.org/10.1023/A:1015062925570