Abstract
An efficient transformation protocol was established for Chinese foxglove, Rehmannia glutinosa L., using Agrobacterium tumefaciens. Putative transgenic plants were induced from leaf explants co-cultivated with Agrobacterium. Detection of the neomycin phosphotransferase gene, high activity of β-glucuronidase (GUS) transcripts and histochemical localization of GUS confirmed the integrative transformation of Chinese foxglove. This result demonstrates the potential for using A. tumefaciens to transfer important genes into commercial Chinese foxglove cultivars.
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Park, SU., Kim, HH., Yu, CY. et al. Agrobacterium-mediated genetic transformation of Chinese foxglove, Rehmannia glutinosa L.. Biotechnology Letters 24, 547–550 (2002). https://doi.org/10.1023/A:1014860424371
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DOI: https://doi.org/10.1023/A:1014860424371