Abstract
Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation.
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Wong, V., Williams, D. & Colby, C.B. Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects. Biotechnology Letters 23, 1841–1847 (2001). https://doi.org/10.1023/A:1012766116568
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DOI: https://doi.org/10.1023/A:1012766116568