Abstract
We demonstrate that an oscillatory flow reactor is a viable reactor for protein refolding via direct dilution. The mixing characteristics of the oscillatory flow reactor are well described and controllable and, importantly, can be scaled-up to process scale without a loss of mixing efficiency. This makes the oscillatory flow reactor an attractive alternative to conventional stirred-tank reactors for process-scale renaturation.
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Lee, C., Mackley, M., Stonestreet, P. et al. Protein refolding in an oscillatory flow reactor. Biotechnology Letters 23, 1899–1901 (2001). https://doi.org/10.1023/A:1012734214751
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DOI: https://doi.org/10.1023/A:1012734214751