Abstract
Using a mechanism of `self-generation', polymer surfaces were coated with ocular mucin-type glycoproteins that were extracted from tear fluid and immobilized through specific interaction with a lectin, jacalin. Separately, jacalin affinity chromatography of tear fluid showed the main retained components had molecular weights higher than 200 kDa. In evaluations of bacterial adhesion, a model surface with jacalin-immobilized ocular mucins took up a significantly smaller number of adhered Staphylococcus epidermidis (0.041×106 cells cm−2) than a bare surface of the same polymer (1.202×106 cells cm−2). The lectin-mediated ocular mucin coating reduced the bacteria uptake by about 95% showing that the presence of mucin on surfaces may afford a general protection against bacterial colonization.
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Shi, L., Ardehali, R., Valint, P. et al. Bacterial adhesion to a model surface with self-generated protection coating of mucin via jacalin. Biotechnology Letters 23, 437–441 (2001). https://doi.org/10.1023/A:1010370205120
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DOI: https://doi.org/10.1023/A:1010370205120