Abstract
Of 60 different thermophilic enrichment cultures, 16 converted glycerol anaerobically to 1,3-propanediol. Two PD-forming strains were further enriched, isolated, and characterised. For the most active strain, AT1, the optimal cultivation parameters for pH and temperature were determined as 5.8 to 6.0 and 58 °C, respectively. In batch-fermentations with AT1, 6.4 g propanediol per litre was formed with a productivity of 0.17 g l−1 h−1.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abbad-Andaloussi S,Guedon E,Spiesser E,Petitdemange H (1996) Glycerol dehydratase activity: the limiting step for the 1,3–propanediol production by Clostridium butyricum DSM 5431. Lett. Appl. Microbiol. 22: 311–314.
Biebl H,Menzel K,Zeng AP,Deckwer WD (1999) Microbial production of 1,3–propanediol. Appl. Microbiol. Biotechnol. 52: 289–297.
Canganella F,Wiegel J (1993) The potential of thermophilic clostridia in biotechnology. In: Woods DR, ed. The Clostridia in Biotechnology. Stoneham: Butterworth, pp. 393–429.
Canganella F,Wiegel J (2000) Continuous cultivation of Clostridium thermobutyricum in a rotary fermentor system. J. Ind. Microbiol. Biotechnol. 24: 7–13.
Deckwer WD (1995) Microbial conversion of glycerol to 1,3–propanediol. FEMS Microbiol. Rev. 16: 143–149.
DSMZ (2000) German Collection of Microorganisms and Cell Cultures, List of media at URL: http://www.dsmz.de/media/media.htm
Papanikolaou S,Ruiz-Sanchez P,Pariset B,Blanchard F,Fick M (2000) High production of 1,3–propanediol from industrial glycerol by newly isolated Clostridium butyricum strain. J. Biotechnol. 77: 191–208.
Reimann A,Biebl H (1996) Production of 1,3–propanediol by Clostridium butyricum DSM 5431 and product tolerant mutants in fedbatch culture: feeding strategy for glycerol and ammonium. Biotechnol. Lett. 18: 827–832.
Reimann A,Biebl H,Deckwer WD (1998) Production of 1,3–propanediol by Clostridium butyricum in continuous culture with cell recycling. Appl. Microbiol. Biotechnol. 49: 359–363.
Wiegel J,Seung-Uk K,Kohring GW (1989) Clostridium thermobutyricum sp. nov., a moderate thermophile isolated from a cellulolytic culture, that produces butyrate as the major product. Int. J. Syst. Bacteriol. 39: 199–204.
Wittlich P,Schlieker M,Jahnz U,Willke T,Vorlop KD (1999) Bioconversion of raw glycerol to 1,3–propanediol by immobilized bacteria. In: Proceedings Ninth European Congress on Biotechnology, July 11–15, Brussels, no. P2762, ISBN 805215–1–5.
Zeng AP,Ross A,Biebl H,Tag C,Günzel B,Deckwer WD (1994) Multiple product inhibition and growth modelling of Clostridium butyricum and Klebsiella pneumoniae in glycerol fermentation. Biotechnol. Bioeng. 44: 902–911.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wittlich, P., Themann, A. & Vorlop, KD. Conversion of glycerol to 1,3-propanediol by a newly isolated thermophilic strain. Biotechnology Letters 23, 463–466 (2001). https://doi.org/10.1023/A:1010329321185
Issue Date:
DOI: https://doi.org/10.1023/A:1010329321185