Abstract
The reaction of poly(U) hydrolysis catalyzed by binase while the latter is inhibited by barstar has been investigated. The inhibition constant for barstar and the apparent Michaelis constants for the inhibition by barstar in the presence of ethanol and NaCl have been determined. Both ethanol and NaCl enhance the inhibition by barstar. This suggests that the binding of barstar with binase is probably due to the interaction of hydrophobic sites rather than by electrostatic interaction between amino acid residues.
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REFERENCES
Hartley, R. W. (1993) Biochemistry, 32, 5978–5984.
Buckle, A. M., Schreiber, G., and Fersht, A. R. (1994) Biochemistry, 33, 8878–8889.
Yakovlev, G. I., Moiseyev, G. P., Protasevich, I. I., Ranjbar, B., Kirpichnikov, M. P., Gilli, R. M., Briand, C. M., Hartley, R., and Makarov, A. A. (1995) FEBS Lett., 366, 156–158.
Shulga, A. A., Okorokov, A. L., Panov, K. I., Kurbanov, O. T., Chernov, B. K., Skryabin, K. G., and Kirpichnikov, M. P. (1994) Mol. Biol. (Moscow), 28, 453–463.
Lubienski, M. J., Bycroft, M., Freund, S. M. V., and Fersht, A. R. (1994) Biochemistry, 33, 8866–8877.
Yagodina, L. O., Altshuler, A. E., and Abreimova, Yu. V. (1999) Biochemistry (Moscow), 64, 957–961.
Golubenko, I. A., Balaban, N. P., Leshchinskaya, I. B., Volkova, T. I., Kleiner, G. I., Chepurnova, N. K., Afanasenko, G. A., and Dudkin, S. M. (1979) Biokhimiya, 44, 640–648.
Akhadov, Ya. Yu. (1977) Dielectric Properties of Binary Solutions [in Russian], Nauka, Moscow.
Stupishina, E. A., and Vershinina, V. I. (1998) Biochemistry (Moscow), 63, 1277–1281.
Yakovlev, G. I., Moiseyev, G. P., Struminskaya, N. K., Borzykh, O. A., Kipenskaya, L. V., Znamenskaya, L. V., Leshchinskaya, I. B., Chernokalskaya, E. B., and Hartley, R. (1994) FEBS Lett., 354, 305–306.
Shulga, A., Kurbanov, F., Kirpichnikov, M., Protasevich, I., Lobachev, V., Ranjbar, B., Chekhlov, V., Polyakov, K., Engelborghs, Y., and Makarov, A. (1998) Protein Eng., 11, 775–782.
Yakovlev, G. I., Chepurnova, N. K., Moiseyev, G. P., Bocharov, A. L., and Lopatnev, S. V. (1987) Bioorg. Khim., 13, 338–343.
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Yagodina, L.O. Interaction of the Bacterial Ribonuclease Binase with the Polypeptide Inhibitor Barstar Based on Kinetic Data on Poly(U) Hydrolysis. Biochemistry (Moscow) 66, 319–322 (2001). https://doi.org/10.1023/A:1010260115449
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DOI: https://doi.org/10.1023/A:1010260115449