Abstract
Metabolically-active mycelia of Penicillium sp. PT1 took up Zn2+ in a biphasic mode, involving an initial energy-dependent binding of Zn2+ to the cell surface, followed by a slower intracellular accumulation. The independent binding probably involved a simple ion exchange, as indicated by the pH decrease during the initial adsorption from 4.55 to 3.28. Intracellular accumulation probably involved polyphosphate precipitation as suggested by transmission electron microscopy
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Aiking H, Stijnman A, Garderen C, Heerikhuizen H, Riet J (1984) Inorganic phosphate accumulation and cadmium detoxification in Klebsiella aerogenes NCTC 418 growing in continuous culture. Appl. Environ. Microbiol. 47: 374–377.
Boswell CD, Dick RE, Macaskie LE (1995) Toxicity of heavy metal to the polyphosphate metabolism of Acinetobacter spp. In: Jerez CA, Vargas T, Toledo H, Wiertz JV, eds. Biohydrometallurgical Processing,Vol. 2. Chile: Talleres del Depto de Estudios Humanisitcos, pp. 299–306.
Chen P, Ting YP (1995) Effect of heavy metal uptake on the electrokinetic properties of Saccharomyces cerevisiae. Biotechnol. Lett. 17: 107–112.
Fourest E, Roux JD (1992) Heavy metal biosorption by fungal mycelial by products: Mechanisms and influence of pH. Appl. Microbiol. Biotechnol. 37: 399–403.
Gadd GM (1990) Heavy metal accumulation by bacteria and other microorganism. Experientia 46: 834–840.
Gadd GM (1994) Interactions of fungi with toxic metals. In: Powell KA, Renwick A, eds. The Genus Aspergillus From Taxonomy and Genetics to Industrial Application. New York, Renwick: Plenum Press, pp. 361–374.
Macaskie LE, Dean ACR, Cheetham AK, Jakeman RJB, Skamulis AJ (1987) Cadmium accumulation by a Citrobacter sp.: the chemical nature of the accumulation metal precipitation and its location on the bacterial cells. J. Gen. Microbiol. 133: 539–544.
Mago R, Srivastava S (1994) Uptake of zinc in Pseudomonas sp. strain UDG26. Appl. Environ. Microbiol. 60: 2367–3270.
Mehra K, Winge DR (1991) Metal ion resistance in fungi: molecular mechanisms and their regulated expression. J. Cell. Biochem. 45: 30–40.
Rao NN, Roberts MF, Torriani A (1985) Amount and chain length of polyphosphates in Escherichia coli depend on cell growth conditions. J. Bacteriol. 162: 242–247.
Remacel J, Vercheval C (1991) A zinc-binding protein in a metalresistance strain, Alcaligenes eutrophus CH34. Can. J. Microbiol. 37: 875–877.
Silver S, Phung LeT (1996) Bacterial heavy metal resistance: new surprises. Ann. Rev. Microbiol. 50: 753–789.
Tantiwachwuttikul P, Dudeney AWL, Leak DJ (1993) Mechanism for the development of nickel tolerance in Penicillium simplicissmum involved in microbial leaching. In: Torma AE, Wey JE, Laskshmanan VI, eds. Biohydrometallurgical Technologies, Vol. 1. Pennsylvania: Warrendale, pp. 363–372.
Tobin JM, Copper DG, Neufeld RJ (1984) Uptake of metal ions by Rhizopus arrhizus biomass. Appl. Environ. Microbiol. 47: 821–824.
Tungkaviveshkul T, Thiravetyan P, Tanticharoen M (1995) Mechanism for bioleaching of zinc silicate residue by organic acid producing microorganisms. In: Vargas T, Jerez CA, Wiertz JV, Toledo H, eds. Biohydrometallurgical Processing, Vol. 1. Chile: Talleres del Depto do Estudios Humanisitcos, pp. 385–393.
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Sintuprapa, W., Thiravetyan, P. & Tanticharoen, M. A possible mechanism of Zn2+ uptake by living cells of Penicillium sp.. Biotechnology Letters 22, 1709–1712 (2000). https://doi.org/10.1023/A:1005688132205
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DOI: https://doi.org/10.1023/A:1005688132205