Abstract
DAF (2,7-diaminofluorene)-peroxidases were the principal oxidative system in roots and rhizosphere of lucerne. The expression of these enzymes was stimulated by mycorrhiza as well as by anthracene (500 mg kg−1 added to the growth medium). Inversely, infection by mycorrhizal fungi repressed laccase activities. Electrophoretic analysis of the DAF-peroxidases revealed similar isoenzyme patterns for all treatments but in presence of anthracene some of the isoenzymes had more intensive bands.
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Criquet S, Farnet AM, Tagger S, Le Petit J (2000) Annual variations of phenoloxidase activities in an evergreen oak litter: influence of certain biotic a abiotic factors. Soil Biol. Biochem.(in press)
Criquet S, Tagger S, Vogt G, Iacazio G, Le Petit J (1999) Laccase activity of forest litter. Soil Biol. Biochem. 31: 1239–1244.
Eggen T, Sveum P (1999) Decontamination of aged creosote polluted soil: the influence of temperature, white rot fungus Pleurotus ostreatus, and pretreatment. Int. Biodeterior. Biodegrad. 43: 125–133.
Fieldes MA, Gerhardt KE (1998) Flax guaiacol peroxidases can be used to illustrate the possibility of misinterpreting the effects of stress on the activity of developmentally regulated enzymes. Plant. Sci. 132: 89–99.
Gianinazzi-Pearson V, Dumas-Gaudot E, Gollotte A, Tahiri-Alaoui A, Gianinazzi S (1996) Cellular and molecular defence-related root responses to invasion by arbuscular mycorrhizal fungi. New Phytol. 133: 45–57.
Gramss G, Rudeschko O (1998) Activities of oxidoreductases enzymes in tissue extracts and sterile root exudates of three crop plants, and some properties of the peroxidase component. New Phytol. 138: 401–409.
Gramss G, Kirsche B, Voigt KD, Günther T, FritscheW(1999) Conversion rates of five polycyclic aromatic hydrocarbons in liquid cultures of fifty eight fungi and the concomitant production of oxidative enzymes. Mycol. Res. 103: 1009–1018.
Günther T, Dornberger U, Fritsche W (1996) Effect of ryegrass on biodegradation of hydrocarbons in soil. Chemosphere 33: 203–215.
Joner EJ, Leyval C (2000) Arbuscular mycorrhizal influence on clover and ryegrass grown together in a soil spiked with polycyclic aromatic hydrocarbons. Mycorrhiza(in press)
Joner EJ, Magid J, Gahoonia TS, Jakobsen I (1995) P depletion and activity of phosphatases in the rhizosphere of mycorrhizal and non-mycorrhizal cucumber (Cucumis sativus L.) Soil Biol. Biochem. 27: 1145–1151.
Kormanik PP, McGraw AC (1982) Methods and principles in mycorrhizal research. In: Schenck NC (ed.), Am. Phytopath. Soc. St Paul, pp 37–45.
Kraus JJ, Munir IZ, McEldoon JP, Clark DS, Dordick JS (1999) Oxidation of polycyclic aromatic hydrocarbons catalyzed by soybean peroxidase. Appl. Biochem. Biotech. 80: 221–230.
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
Leyval C, Binet P (1998) Effect of polyaromatic hydrocarbons in soil on arbuscular mycorrhizal plants. J. Environ. Qual. 27: 402–407.
McArthur DAJ, Knowles NR (1992) Resistance responses of potato to vesicular-arbuscular mycorrhizal fungi under varying abiotic phosphorus levels. Plant Physiol. 100: 341–351.
Münzenberger B, Otter T, Wüstrich D, Polle A (1997) Peroxidase and laccase activities in mycorrhizal and non-mycorrhizal fine roots of Norway spruce (Picea abies) and larch (Larix decidua) Can. J. Bot. 75: 932–938.
Rama R, Mougin C, Boyer FD, Kollmann A, Malosse C, Sigoillot PJC (1998) Biotransformation of benzo[a]pyrene in bench scale reactor using laccase of Pycnoporus cinnabarinus. Biotechnol. Lett. 12: 1101–1104.
Rama-Mercier R, Mougin C, Sigoillot JC, Sohier L, Chaplain V, Asther M (1998) Wet sand cultures to screen filamentous fungi for the biotransformation of polycyclic aromatic hydrocarbons. Biotechnol. Tech. 12: 725–728.
Reilley KA, Banks MK, Schwab AP (1996) Dissipation of polycyclic aromatic hydrocarbons in the rhizosphere. J. Environ. Qual. 25: 212–219.
Salzer P, Corbiere H, Boller T (1999) Hydrogen peroxide accumulation in Medicago truncatula roots colonized by the arbuscular mycorrhiza-forming fungus Glomus intraradices. Planta 208: 319–325.
Smith SE, Read DJ (1997) Mycorrhizal Symbiosis.San Diego: Academic Press.
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Criquet, S., Joner, E., Leglize, P. et al. Anthracene and mycorrhiza affect the activity of oxidoreductases in the roots and the rhizosphere of lucerne (Medicago sativa L.). Biotechnology Letters 22, 1733–1737 (2000). https://doi.org/10.1023/A:1005604719909
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DOI: https://doi.org/10.1023/A:1005604719909