Abstract
Siliceous sponges (Hexactinellida and Demospongiae classes) are aquatic invertebrates which are important both for marine and freshwater ecology and also as the source of biologically active compounds. The sponge skeleton consists of spicules - needle-like or branched composite structures based on silicon dioxide. Mechanisms of silicon assimilation and synthesis of high-ordered glass-like structures at ambient temperatures by sponges are intriguing for biologists, chemists and nanotechnologists. Fluorescent amines are in-vivo dyes that stain growing siliceous frustules of diatom algae so the use of these agents for the sponge study was attempted. We found that cultivation of the Lubomirskia baicalensis (Pallas, 1773) sponge in the presence of fluorescent tracers of biosilica - N1,N3,N3-trimethyl-N1-(7-nitro-2,1,3- benzoxadiazol-4-yl)propane-1,3-diamine and N1,N3-dime thyl-N1-[3-(dimethylamino)propyl]-N3-(7-nitro-2,1,3-benz oxadiazo-4yl)propane-1,3-diamine results in the staining of growing siliceous spicules. This finding shows that amine dyes accompany silicon from the environment to sponges spicules which opens a new way to study of silicon assimilation by sponges. Fluorescent staining of the growing spicules following with the confocal microscopy can be a powerful tool for morphological studies, revealing information about the dynamics of spiculogenesis and for bio-fabrication of new fluorescent materials.
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References
Borchiellini C, Chombard C, Lafay B, Boury-Esnault N (2000) Molecular systematics of sponges (Porifera). Hydrobiologia 420:15–27
Wang X, Schröder H C, Wang K, Kaandorpd J A, Müller W E G (2012) Genetic, biological and structural hierarchies during sponge spicule formation: from soft sol–gels to solid 3D silica composite structures. Soft Matter 8:9501–9518
Blunt J W, Copp B R, Keyzers R A, Munro M H G, Prinsep M R (2013) Marine natural products. Nat Prod Rep 30:237–323
Uriz M J, Turon X, Becerro M A, Agell G (2003) Siliceous spicules and skeleton frameworks in sponges: origin, diversity, ultrastructural patterns, and biological functions. Microsc Res Tech 62:279–299
Uriz M J, Turon X, Becerro M A (2003) Silica deposition in demosponges. Progr Mol Subcell Biol 33:163–193
Weaver J C, Pietrasanta L I, Hedin N, Chmelka B F, Hansma P K, Morse D E (2003) Nanostructural features of demosponge biosilica. J Struct Biol 144:271–281
Lopez P J, Gautiera C, Livageband J, Coradin T (2005) Mimicking biogenic silica nanostructures formation. Curr Nanosci 1:73–83
Ehrlich H, Janussen D, Simon P, Bazhenov V V, Shapkin N P, Erler C, Mertig M, Born R, Heinemann S, Hanke T, Worch H, Vournakis J N (2008) Nanostructural organization of naturally occurring composites—part II: silica-chitin-based biocomposites. J Nanomater Artic ID 670235:1–8. 10.1155/2008/670235
Gröger C, Lutz K, Brunner E (2008) Biomolecular self-assembly and its relevance in silica biomineralization. Cell Biochem Biophys 50:23–39
Schröder H C, Wang X, Tremel W, Ushijima H, Müller. W E G (2008) Biofabrication of biosilica-glass by living organisms. Nat Prod Rep 25:455–474
Müller W E G, Wang X, Cui F-Z, Jochum P K, Tremel W, Bill J, Schröder H C, Natalio F, Schloßmacher U, Wiens M, Sponge spicules as blueprints for the biofabrication of inorganic–organic composites and biomaterials (2009). Appl Microbiol Biotechnol 83:397–413
Mayer G (2009) Role of biosilica in materials science: lessons from siliceous biological systems for structural composites. Progr Mol Subcell Biol 47:277–294
Asmathunisha N, Kathiresan K (2013) A review on biosynthesis of nanoparticles by marine organisms. Colloids Surf. B Biointerfaces 103:283–287
Li C-W, Chu S, Lee M (1989) Characterizing the silica deposition vesicle of diatoms. Protoplasma 151:158–163
Brzezinski M A, Conley D J (1994) Silicon deposition during the cell cycle of Thalassiosira weissflogii (Bacillariophyceae) determined using dual rhodamine 123 and propidium iodide staining. J Phycol 30:45–55
Shimizu K, Del Amo Y, Brzezinski M A, Stucky G D, Morse D E (2001) A novel fluorescent silica tracer for biological silification studies. Chem Biol 8:1051–1060
Hazelaar S, Van Der Strate H J, Gieskes W W C, Vrieling E G (2005) Monitoring rapid valve formation in the pennate diatom Navicula salinarum (Bacillariophyceae). J Phycol 41:54–58
Desclés J, Vartanian M, AEl Harrak, Quinet M, Bremond N, Sapriel G, Bibette J, Lopez P J (2008) New tools for labeling silica in living diatoms. New Phytol 177:822–829
Kucki M (2009) Biological photonic crystals: diatoms dye functionalization of biological silica nanostructures. University of Kassel, Dissertation
Kucki M, Fuhrmann-Lieker T (2012) Staining diatoms with rhodamine dyes: control of emission colour in photonic biocomposites. J R Soc Interface 9:727–733
Tesson B, Hildebrand M (2010) Extensive and intimate association of the cytoskeleton with forming silica in diatoms: control over patterning on the meso- and micro-scale. PLoS ONE 5:e14300
Ichinomiya M, Gomi Y, Nakamachi M, Ota T, Kobari T (2010) Temporal patterns in silica deposition among siliceous plankton during the spring bloom in the Oyashio region. Deep-Sea Res Pt II 57:1665–1670
Ogane K, Tuji A, Suzuki N, Matsuoka A, Kurihara T, Hori R S (2010) Direct observation of the skeletal growth patterns of polycystine radiolarians using a fluorescent marker. Mar Micropaleontol 77:137–144
Aizenberg J, Sundar V C, Yablon A D, Weaver J C, Chen G (2004) Biological glass fibers: Correlation between optical and structural properties. PNAS 101:3358–3363
Kulchin Yu N, Bezverbny A V, Bukin O A, Voznesensky S S, Galkina A N, Drozdov A L, Nagorny I G (2009) Optical and nonlinear optical properties of sea glass sponge spicules. Progr Mol Subcell Biol 47:315–340
Annenkov V V, Danilovtseva E N, Zelinskiy S N, Basharina T N, Safonova T A, Korneva E S, Likhoshway YeV, Grachev M A (2010) Novel fluorescent dyes based on oligopropylamines for the in vivo staining of eukaryotic unicellular algae. Anal Biochem 407:44–51
Annenkov V V, Basharina T N, Danilovtseva E N, Grachev M A (2013) Putative silicon transport vesicles in the cytoplasm of the diatom Synedra acus during surge uptake of silicon. Protoplasma
Sumper M, Brunner E, Lehmann G (2005) Biomineralization in diatoms: characterization of novel polyamines associated with silica. FEBS Lett 579:3765–3769
Matsunaga S, Sakai R, Jimbo M, Kamiya H (2007) Long-chain polyamines (LCPAs) from marine sponge: possible implication in spicule formation. ChemBioChem 8:1729–1735
Schröder H-C, Perović-Ottstadt S, Rothenberger M, Wiens M, Schwertner H, Batel R, Korzhev M, Müller IM, Müller WEG (2004) Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter. Biochem J 381:665–673
Efremova S M (2001) In: Timoshkin OA ed Index of animal species inhabiting Lake Baikal and its catchment area. Nauka, Novosibirsk
Glyzina O Yu, Glyzin A V, Lubotchko S A (2011) Investigation of Baikal hydrosimbionts with pilot aquarian complexes. Water: Chem Ecol 2:35–40. http://watchemec.ru/en/article/23449/
Glyzina O Yu, Glyzin A V, Sukhanova L V, Tyagun M L, Sapojnikova Yu P, Belykh O I, Dzyuba E V, Zaitseva A N, Kulikov V A (2012) Cold-water freshwater aquarian complex for scientific investigations. Water: Chem Ecol 12:78–86. http://watchemec.ru/article/25263/
Suturin A N, Paradina L F, Epov V N, Semenov A R, Lozhkin V I (2002) Development of a standard sample of composition of deep Baikalian water. Chem Sustain Dev 10:473–482
Shimizu K, Cha J, Stucky G D, Morse D E (1998) Silicatein α: Cathepsin L-like protein in sponge biosilica. PNAS 95:6234–6238
Ingalls A E, Whitehead K, Bridoux M C (2010) Tinted windows: The presence of the UV absorbing compounds called mycosporine-like amino acids embedded in the frustules of marine diatoms. Geochim Cosmochim Acta 74:104–115
Friedrichs L (2013) A simple cleaning and fluorescent staining protocol for recent and fossil diatom frustules. Diatom Res 28:317–327
Manconi R, Pronzato R (2002) In: Hooper JNA, Van Soest RWM eds Systema Porifera. A guide to the classification of sponges. Kluwer Academic/Plenum Publishers, Dordrecht
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Annenkov, V.V., Glyzina, O.Y., Verkhozina, O.N. et al. Fluorescent Amines as a New Tool for Study of Siliceous Sponges. Silicon 6, 227–231 (2014). https://doi.org/10.1007/s12633-014-9220-4
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DOI: https://doi.org/10.1007/s12633-014-9220-4