Abstract
We examined the effects of crude oil contamination on community assemblages of meiofauna and nematodes after exposure to total petroleum hydrocarbons in the laboratory. We administered a seawater solution that had been contaminated with total petroleum hydrocarbons to seven treatment groups at different concentrations, while the control group received uncontaminated filtered seawater. The average density of total meiofauna in the experimental microcosms diluted with 0.5%, 1%, 2%, and 4% contaminated seawater was higher than the density in the control. The average density of total meiofauna in the 8%, 15%, and 20% microcosms was lower than the density in the control. The density of nematodes was similar to that of the total meiofauna. Cluster analysis divided the microcosms into group 1 (control, 0.5%, 1%, 2%, and 4% microcosms) and group 2 (8%, 15%, and 20% microcosms). However, SIMPROF analysis showed no significant difference between the two groups (p > 0.05). Bolbolaimus spp. (37.1%) were dominant among the nematodes. Cluster analysis showed similar results for nematode and meiofaunal communities. The total meiofaunal density, nematode density, and number of Bolbolaimus spp. individuals were significantly negatively associated with the concentration of total petroleum hydrocarbons (Spearman correlation coefficients, p < 0.05). Within the nematodes, epistrate feeders (group 2A: 46%) were the most abundant trophic group. Among the treatment groups, the abundance of group 2A increased in low-concentration microcosms and decreased in high-concentration microcosms. Thus, our findings provide information on the effects of oil pollution on meiofauna in the intertidal zones of sandy beaches.
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References
Ansari ZA, Ingole B (2002) Effect of an oil spill from M V Sea Transporter on intertidal meiofauna at Goa, India. Mar Pollut Bull 44:396–402
Ansari ZA, Parulekar AH (1993) Distribution, abundance and ecology of the meiofauna in a tropical estuary along the west coast of India. Hydrobiologia 262:115–126
Atlas RM (1995) Bioremediation of petroleum pollutants. Int. Biodeterior. Biodegradation 35:317–327
Austen MC (1989) Factors affecting estuarine meiobenthic assemblage structure: a multifactorial microcosm experiment. J Exp Mar Biol Ecol 130:167–187
Austen MC, McEvoy AJ (1997) The use of offshore meiobenthic communities in laboratory microcosm experiments: response to heavy metal contamination. J Exp Mar Biol Ecol 211:247–261
Austen MC, McEvoy AJ, Warwick RM (1994) The specificity of meiobenthic community response to different pollutants: results from microcosm experiments. Mar Pollut Bull 28:557–563
Austen MC, Somerfield PJ (1997) A community level sediment bioassay applied to an estuarine heavy metal gradient. Mar Environ Res 43:315–328
Beyrem H, Aissa P (2000) Les nematodes libres, organisms sentinelles de l’evolution des concentrations d’hydrocarbures dans la baie de Bizerte (Tunisie). Cah Biol Mar 41:329–342
Bodin P (1988) Results of ecological monitoring of three beaches polluted by the Amoco Cadiz oil spill: development of meiofauna from 1978 to 1984. Mar Ecol-Prog Ser 42:105–123
Boesch DB, Rosenberg R (1981) Response to stress in marine benthic communities. In: Barrett GW, Rosenberg R (eds) Stress effects on natural ecosystems. John Wiley & Sons Ltd., Chichester, pp 179–214
Bongers T (1990) The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83:14–19
Bongers T, Alkemade R, Yeates GW (1991) Interpretation of disturbance-induced maturity decrease in marine nematode assemblages by means of the Maturity Index. Mar Ecol-Prog Ser 76:135–142
Burgess R (2001) An improved protocol for separating meiofauna from sediments using colloidal silica sols. Mar Ecol-Prog Ser 214:161–165
Boucher G (1980). Impact of Amoco Cadiz oil spill on intertidal and sublittoral meiofauna. Mar Pollut Bull 11:95–101
Bouwman LA, Romeijn K, Admiraal W (1984) On the ecology of meiofauna in an organically polluted estuarine mudflat. Estuar Coast Shelf S 19:633–653
Carman KR, Fleeger JW, Means JC, Pomarico SM, McMillin DJ (1995) Experimental investigation of polynuclear aromatic hydrocarbons on an estuarine sediment food web. Mar Environ Res 40:298–318.
Carman KR, Fleeger JW, Pomarico SM (2000) Does historical exposure to hydrocarbon contamination alter the response of benthic communities to diesel contamination? Mar Environ Res 49(3):255–278
Clarke KR, Gorley RN (2001) PRIMER v.5. User manual. PRIMER-E, Plymouth, 91 p
Commito JA, Tita G (2002) Differential dispersal rates in an intertidal meiofauna assemblage. J Exp Mar Biol Ecol 268: 237–256
Coull BC, Chandler GT (1992) Pollution and meiofauna: field, laboratory and mesocosm studies. Oceanogr Mar Biol 30: 191–271
Danovaro R (2000) Benthic microbial loop and meiofaunal response to oil-induced disturbance in coastal sediments: a review. Int J Environ Pollut 13:380–391
Danovaro R, Fabiano M, Vincx M (1995) Meiofauna response to the Agip Abruzzo oil spill in subtidal sediments of the Ligurian Sea. Mar Pollut Bull 30(2):133–145
Dauvin JC (1982) Impact of Amoco Cadiz oil spill on the muddy fine sand Abra alba and Melinna palmata community from the Bay of Morlaix. Estuar Coast Shelf S 14:517–531
Edgar GJ, Barrett NS (2000) Impact of the Iron Baron oil spill on subtidal reef assemblages in Tasmania. Mar Pollut Bull 40:36–49
Elmgren R, Hansson S, Larsson U, Sundelin B, Boehm PD (1983) The ‘Tsesis’ oil spill: acute and long-term impact on the benthos. Mar Biol 73:51–65
Feder HM, Naidu AS, Paul AJ (1990) Trace metal and biotic changes following a simulated oil spill on a mudflat in Port Valdez, Alaska. Mar Pollut Bull 21:131–137
Ferris H, Bongers T (2009) Indices developed specifically for analysis of nematode assemblages. In: Wilson MJ, Kakouli-Duarte T (eds) Nematodes as environmental indicators. CAB International, Wallingford, pp 124–145
Fleeger JW, Chandler GT (1983) Meiofauna response to an experimental oil spill in a Louisiana salt marsh. Mar Ecol-Prog Ser 11:257–264
Friethsen JB, Elmgren R, Rudnick DT (1985) Responses of benthic meiofauna to long-term, low-level additions of No. 2 fuel oil. Mar Ecol-Prog Ser 23:1–14
Gee JM, Warwick RM, Schaaning M, Berge JA, Ambrose Jr. WG (1985) Effects of organic enrichment on meiofaunal abundance and community structure in sublittoral soft sediments. J Exp Mar Biol Ecol 91:247–262
Giere O (1979) The impact of oil pollution on intertidal meiofauna — field studies after the La Coruna-Spill, May 1976. Cah Biol Mar 20(2):231–251
Giere O (1993) Meiobenthology: the microscopic fauna in aquatic sediments. Springer-Verlag Berlin, Heidelberg, New York, 328 p
Higgins RP, Thiel H (1988) Introduction to the study of meiofauna. Smithsonian Institution Press, Washington DC, 488 p
ITOPF (2014) Technical Information Paper TIP 02: fate of marine oil spills. http://www.itopf.com/knowledgeresources/documentsguides/document/tip-2-fate-of-marine-oil-spills Accessed 27 July 2014
Jense P (1987) Feeding ecology of free-living aquatic nematodes. Mar Ecol-Prog Ser 35:187–196
Jewett SC, Dean TA, Smith RO, Blanchard A (1999) ‘Exxon Valdez’ oil spill: impacts and recovery in the soft-bottom benthic community in and adjacent to eelgrass beds. Mar Ecol-Prog Ser 185:59–83
Kennish MJ (1992) Polynuclear aromatic hydrocarbons. In: Kennish MJ (ed) Ecology of estuaries: anthropogenic effects. CRC Press, Boca Raton, pp 133–181
Kang TW, Min WG, Rho HS, Pack HS, Kim DS (2014) Differential responses of a benthic meiofaunal community to an artificial oil spill in the intertidal zone. J Mar Biol Assoc UK 94(2):219–231
Kim M, Hong SH, Won J, Yim UH, Jung JH, Ha SY, An JG, Joo C, Kim E, Han GM, Baek S, Choi HW, Shim WJ (2013) Petroleum hydrocarbon contaminations in the intertidal seawater after the Hebei Spirit oil spill-effect of tidal cycle on the TPH concentrations and the chromatographic characterization of seawater extracts. Water Res 47:758–768
KIOST (2009) Annual Report on the environmental impact assessment and environmental restoration of the Hebei Spirit oil spill. A Report Prepared for the Ministry of Land, Transport and Maritime Affairs, Report No. 11-1611000-000392-01, 38 p
KIOST (2015) Annual Report on the environmental assessment and restoration from oil pollution. A Report Prepared for the Ministry of Oceans and Fisheries, Report No. 11-1192000-000438-01, 65 p
Losi V, Montefalcone M, Moreno M, Giovannetti E, Gaozza L, Grondona M, Albertelli G (2012) Nematodes as indicators of environmental quality in seagrass (Posidonia oceanica) meadows of the NW Mediterranean Sea. Adv Oceanogr Limnol 3:69–91
Louati A, Elleuch B, Kallel M, Saliot A, Dagaut J, Oudot J (2001) Hydrocarbon contamination of coastal sediments from the Sfax area (Tunisia), Mediterranean Sea. Mar Pollut Bull 42:445–452
Mahmoudi E, Essid N, Beyrem H, Hedfi A, Boufahja F, Vitiello P, Aissa P (2005) Effects of hydrocarbon contamination on a free living marine nematode community: results from microcosm experiments. Mar Pollut Bull 50:1197–1204
Millward RN, Grant A (1995) Assessing the impact of copper on nematode communities from a chronically metal-enriched estuary using pollution-induced community tolerance. Mar Pollut Bull 30:701–706
Naidu AA, Feder HM, Norrell SA (1978) The effect of Prudhoe Bay crude oil on a tidal-flat ecosystem in Port Valdez, Alaska. In: Proceeding of 10th Offshore Technology Conference, Houston, Texas, pp 97–104
Peterson CH, Kennicutt MC, Green RH, Montagna P, Harper DE, Powell EN, Roscigno PF (1996) Ecological consequences of environmental perturbations associated with offshore hydrocarbon production: a perspective on long-term exposures in the Gulf of Mexico. Can J Fish Aquat Sci 53:2637–2654
Platt HM, Warwick RM (1980) The significance of free-living nematodes to the littoral ecosystem. In: Price JH, Irvine DEG, Farnham WF (eds) The shore environment. Vol. 2. Ecosystems. Academic Press, New York, pp 729–759
Platt HM, Warwick RM (1983) A synopsis of the free-living marine nematodes. Part I. British Enoplids. Cambridge University Press, Cambridge, 306 p
Platt HM, Warwick RM (1988) A synopsis of the free-living marine nematodes. Part II. British Chromadorids. Cambridge University Press, Cambridge, 502 p
Renaud-Mornant J, Gourbault N, de Panafiew JB, Heleouet MN (1981). Effets de la pollution par hydrocarbures sur la meiofauna de la baie de Morlaix. In: Amoco Cadiz, Consequences d’une Pollution Accidentale par les Hydrocarbures. Actes coli. Intern. C.O.B., Brest, France, 19–22 November 1979, pp 551–561
Schratzberger M, Danie F, Wall CM, Kilbride R, Macnaughton SJ, Boyd SE, Rees HL, Swannell RPJ (2003) Response of estuarine meio and macrofauna to in situ bioremediation of oil contaminated sediment. Mar Pollut Bull 46:430–443
Schratzberger M, Warwick RM (1998) Effects of the intensity and frequency of organic enrichment on two estuarine nematode communities. Mar Ecol-Prog Ser 164:84–95
Semprucci F, Losi V, Moreno M (2015) A review of Italian research on free-living marine nematodes and the future perspectives in their use as ecological indicators (EcoInd). Mediterr Mar Sci 16:352–365
Stacey B, Marcotte B (1987) Chronic effect of No. 2 fuel oil on population dynamics of harpacticoid copepods in experimental marine mesocosms. Mar Ecol-Prog Ser 40:61–68
Sundelin B, Elmgren R (1991) Meiofauna of an experimental soft bottom ecosystem-effects of macrofauna and cadmium exposure. Mar Ecol-Prog Ser 70:245–255
Van Damme D, Heip C, Willems KA (1984) Influence of pollution on the harpacticoid copepods of two North Sea estuaries. Hydrobiologia 112:143–160
Warwick RM, Carr MR, Clarke KR, Gee JM, Green RH (1988) A mesocosm experiment on the effects of hydrocarbon and copper pollution on a sublittoral soft-sediment meiobenthic community. Mar Ecol-Prog Ser 46:181–191
Warwick RM, Platt HM, Somerfield PJ (1998) A synopsis of the free-living marine nematodes. Part III. Monhysterids, 53. Field Studies Council, Shrewsbury, 296 p
Wieser W (1953) Die beziehung zwischen Mundhohlengestalt, Ernahrungsweise und Vorkommen bei freilebenden marinen Nematoden. Eine okologisch-morphologische Studie. Ark Zool 4(26):439–484
Wormald AP (1976) Effect of an oil spill of marine diesel oil on meiofauna of a sandy beach at Picnic Bay Hong Kong. Environ Pollut 11:117–130
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Kang, T., Oh, J.H., Hong, JS. et al. Responses of meiofauna and nematode communities to crude oil contamination in a laboratory microcosm experiment. Ocean Sci. J. 51, 465–476 (2016). https://doi.org/10.1007/s12601-016-0042-8
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DOI: https://doi.org/10.1007/s12601-016-0042-8