Abstract
Decellularized human umbilical arteries (dHUA) is an off-the-shelf graft that can potentially serve as vascular scaffolds in tissue engineering of small-diameter vascular grafts. This research aimed to investigate that dHUA could exhibit adequate endothelialization for a long term in xenogenic transplantation. 13 dHUAs were implanted in rat abdominal aortas up to 90 days. Rats were divided into three groups in terms of survival period: Group 1, one to seven days (n = 6); Group 2, 14 to 30 days (n = 4) and Group 3, 90 days (n = 3). The explants were analyzed by histological, immunohistochemistry and magnetic resonance angiography (MRA) examination. Allograft implantation of 12 decellularized rat abdominal aortas’ were processed the same way as the rat in order to make a comparison for survival rates (Group 1, n = 5; Group 2, n = 4; Group 3, n = 3). The results demonstrated that the survival rates of xenograft and allograft implantation were estimated to be 59.2% vs. 58.3% in Group 1, 50.7% vs. 58.3% in Group 2 and 3. Grafts harvested from Group 2 were showed CD31, endothelial nitric oxide synthase expression at intima, and α-smooth muscle actin, CD45, CD68 and CD168 expression at the tunica externa. A layer structure with obvious endothelialization and fiber regeneration/orientation could be inspected from the explants of Group 3. MRA demonstrated the patency of dHUA on day 30 and 90. In conclusion, more than 50% dHUA maintained patency in the xenogenic model till 90 days after surgery. A mature vessel-like functional structure with intact endothelial layer was observed then. This warrants further study in the reinforcement of decellularized vascular scaffolds.
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Acknowledgements
This work was supported by grants R16001 and R16002 from I-MEI FOODS CO., LTD, and Taipei Veterans General Hospital. We thank the Taiwan Mouse Clinic, Academia Sinica and Taiwan Animal Consortium for the technical support in MRA.
Funding
This research received funding support from the Taiwan Ministry of Science and Technology, MOST107-2314-B-075-030, 108-2314-B-075-053, 108-2314-B-075-045, 108WHA0110304, 109WHA0110184, and MOST 109-2314-B-038-143.
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Author’s Contributions
Kai Hsia: Methodology, Data curation, Writing—Original draft preparation & editing. Tien-Shiang Wang: Data curation, Review and editing. Chin-Su Liu: Investigation, Writing—Review and editing. Chih-Kuan Su: Methodology, Original draft preparation. Chien-Chin Chen: Pathology. Chang-Ching Yeh: Sample collection and preparation. Hsinyu Lee: Supervision. Chao-Ling Yao: Supervision. Tsung-Yu Tseng: Administration. Shih-Hwa Chiou: Supervision, Review and editing. Hsu Ma: Supervision. Chih-Hsun Lin: Methodology, Writing—Original draft preparation. Jen-Her Lu: Reviewing and editing, Funding acquisition, Conceptualization and supervision.
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The study was approved by the Institutional Animal Care and Use Committee of Taipei Veterans General Hospital. All animal care complied with the Guide for the Care and Use of Laboratory Animals (No. 2018-265). Human tissue was obtained using protocols approved by the Institutional Review Board of Taipei Veterans General Hospital. All human subjects signed a consent form that was approved by the Institutional Review Board of Taipei Veterans General Hospital (No. 2020-04-006C).
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Hsia, K., Wang, TS., Liu, CS. et al. Decellularized Human Umbilical Artery Exhibits Adequate Endothelialization in Xenogenic Transplantation. Biotechnol Bioproc E 28, 439–450 (2023). https://doi.org/10.1007/s12257-022-0256-9
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DOI: https://doi.org/10.1007/s12257-022-0256-9