Abstract
The development of wound dressings with therapeutical benefits is of great importance in skin tissue engineering applications, adding bioactive molecules into biomaterials is a strategy to achieve a better biological response. In this study, four different concentrations of curcumin (CUR; 5, 10, 15 and 20 by weight in relation to the PCL content) were incorporated into solutions composed of polycaprolactone (PCL) and collagen (COL) for the manufacture of electrospun fibers. The PCL-COL-CUR fibers were physicochemically characterized in terms of their morphology, wettability, degradation rate, mechanical behavior, and cumulative curcumin release. The in vitro biological properties of the composite membranes were also evaluated. The results indicated that the membranes have diameters on average of approximately 200 nm. The water uptake was adequate for exudates remotion in a wound, and the degradation rate of the fibers was highly appropriate to achieve complete skin tissue regeneration. The addition of CUR to composite membranes produced a significant increase in the mechanical properties which indicate a satisfactory clinical handling. The incorporation of CUR produced a significant decrease in the planktonic growth of S. aureus over time, however, the antibacterial effect against E. coli was limited, the presence of CUR did not cause the inhibition of its growth. Finally, the viability of human dermal fibroblasts seeded on the top of the membranes indicated the cytotoxic dosage effect of CUR, the two highest CUR concentrations produced a significant loss of cell viability. Overall, our results suggested that the CUR-loaded PCL-COL composite membranes are promising candidates for use as antibacterial dressings to enhance clinical wound management.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
A. Krishnan and S. Thomas, Polym. Adv. Technol., 30, 823 (2019).
M. Hajialyani, D. Tewari, E. Sobarzo-Sánchez, S. M. Nabavi, M. H. Farzaei, and M. Abdollahi, Int. J. Nanomedicine, 13, 5023 (2018).
A. Gaspar-Pintiliescu, A. M. Stanciuc, and O. Craciunescu, Int. J. Biol. Macromol., 138, 854 (2019).
X. Gao, Z. Xu, G. Liu, and J. Wu, Acta Biomater., 119, 57 (2021).
I. Guimarães, S. Baptista-Silva, M. Pintado, and A. L. Oliveira, Appl. Sci., 11, 1230 (2021).
Y. Zhao and Z. Sun, Int. J. Food Prop., 20, S2822 (2018).
R. R. Reddy, B. V. N. Phani Kumar, G. Shanmugam, B. Madhan, and A. B. Mandal, J. Phys. Chem. B, 119, 14076 (2015).
M. M. Mahmud, S. Zaman, A. Perveen, R. A. Jahan, M. F. Islam, and M. T. Arafat, J. Drug Deliv. Sci. Technol., 55, 101386 (2020).
N. Ahangari, S. Kargozar, M. Ghayour-Mobarhan, F. Baino, A. Pasdar, A. Sahebkar, G. A. A. Ferns, H. W. Kim, and M. Mozafari, BioFactors, 45, 135 (2019).
J. K. Trigo-Gutierrez, Y. Vega-Chacón, A. B. Soares, and E. G. de O. Mima, Int. J. Mol. Sci., 22, 7130 (2021).
M. Ilangovan, V. Guna, C. Hu, G. S. Nagananda, and N. Reddy, Ind. Crops Prod., 112, 556 (2018).
Y. Fan, J. Yi, Y. Zhang, and W. Yokoyama, Food Chem., 239, 1210 (2018).
R. Meng, Z. Wu, Q. T. Xie, J. S. Cheng, and B. Zhang, Food Chem., 340, 127893 (2021).
I. Nakamae, T. Morimoto, H. Shima, M. Shionyu, H. Fujiki, N. Yoneda-Kato, T. Yokoyama, S. Kanaya, K. Kakiuchi, T. Shirai, E. Meiyanto, and J. Y. Kato, Molecules, 24, 1 (2019).
Y. A. Larasati, N. Yoneda-Kato, I. Nakamae, T. Yokoyama, E. Meiyanto, and J. Y. Kato, Sci. Rep., 8, 1 (2018).
N. Fereydouni, M. Darroudi, J. Movaffagh, A. Shahroodi, A. E. Butler, S. Ganjali, and A. Sahebkar, J. Cell. Physiol., 234, 5537 (2019).
E. Blanco-García, F. J. Otero-Espinar, J. Blanco-Méndez, J. M. Leiro-Vidal, and A. Luzardo-Álvarez, Int. J. Pharm., 518, 86 (2017).
T. Esatbeyoglu, K. Ulbrich, C. Rehberg, S. Rohn, and G. Rimbach, Food Funct., 6, 887 (2015).
B. Joe, M. Vijaykumar, and B. R. Lokesh, Crit. Rev. Food Sci. Nutr., 44, 97 (2004).
R. CR, S. PS, O. Manaf, S. PP, and A. Sujith, Int. J. Biol. Macromol., 108, 1261 (2018).
I. Sebe, P. Szabó, B. Kállai-Szabó, and R. Zelkó, Int. J. Pharm., 494, 516 (2015).
S. M. Espinoza, H. I. Patil, E. San Martin Martinez, R. Casañas Pimentel, and P. P. Ige, Int. J. Polym. Mater. Polym. Biomater., 69, 85 (2020).
J. Dulnik, D. Kołbuk, P. Denis, and P. Sajkiewicz, Eur. Polym. J., 104, 147 (2018).
S. R. Gomes, G. Rodrigues, G. G. Martins, M. A. Roberto, M. Mafra, C. M. R. Henriques, and J. C. Silva, Mater. Sci. Eng. C, 46, 348 (2015).
A. Hernández-Rangel and E. S. Martin-Martinez, J. Biomed. Mater. Res. Part A, 109, 1751 (2021).
E. J. Chong, T. T. Phan, I. J. Lim, Y. Z. Zhang, B. H. Bay, S. Ramakrishna, and C. T. Lim, Acta Biomater., 3, 321 (2007).
K. S. Silvipriya, K. Krishna Kumar, B. Dinesh Kumar, A. John, and P. Lakshmanan, Curr. Trends Biotechnol. Pharm., 10, 374 (2016).
Q. Zhang, S. Lv, J. Lu, S. Jiang, and L. Lin, Int. J. Biol. Macromol., 76, 94 (2015).
C. Bi, X. Li, Q. Xin, W. Han, C. Shi, R. Guo, W. Shi, R. Qiao, X. Wang, and J. Zhong, J. Biosci. Bioeng., 128, 234 (2019).
Q. Li, L. Mu, F. Zhang, Y. Sun, Q. Chen, C. Xie, and H. Wang, Mater. Sci. Eng. C, 80, 346 (2017).
T. Zhou, N. Wang, Y. Xue, T. Ding, X. Liu, X. Mo, and J. Sun, Colloids Surfaces B Biointerfaces, 143, 415 (2016).
A. Afifah, O. Suparno, L. Haditjaroko, and K. Tarman, IOP Conf. Ser. Earth Environ. Sci., 335, 012031 (2019).
M. Ghorbani, P. Nezhad-Mokhtari, and S. Ramazani, Int. J. Biol. Macromol., 153, 921 (2020).
Y. E. Aguirre-Chagala, V. Altuzar, E. León-Sarabia, J. C. Tinoco-Magaña, J. M. Yañez-Limón, and C. Mendoza-Barrera, Mater. Sci. Eng. C, 76, 897 (2017).
M. Fallah, S. H. Bahrami, and M. Ranjbar-Mohammadi, J. Ind. Text., 46, 562 (2016).
H. T. Bui, O. H. Chung, J. Dela Cruz, and J. S. Park, Macromol. Res., 22, 1288 (2014).
S. M. Saeed, H. Mirzadeh, M. Zandi, and J. Barzin, Prog. Biomater., 6, 39 (2017).
P. Kittiphattanabawon, S. Benjakul, W. Visessanguan, T. Nagai, and M. Tanaka, Food Chem., 89, 363 (2005).
D. Liu, L. Liang, J. M. Regenstein, and P. Zhou, Food Chem., 133, 1441 (2012).
M. Ahmad and S. Benjakul, Food Chem., 120, 817 (2010).
M. S. Heu, J. H. Lee, H. J. Kim, S. J. Jee, J. S. Lee, Y.-J. Jeon, F. Shahidi, and J.-S. Kim, Food Sci. Biotechnol., 19, 27 (2010).
G. Prado-Prone, P. Silva-Bermudez, M. Bazzar, M. L. Focarete, S. E. Rodil, X. Vidal-Gutiérrez, J. A. García-Macedo, V. I. García-Pérez, C. Velasquillo, and A. Almaguer-Flores, Biomed. Mater., 15, 035006 (2020).
X. Z. Sun, G. R. Williams, X. X. Hou, and L. M. Zhu, Carbohydr. Polym., 94, 147 (2013).
M. Zahiri, M. Khanmohammadi, A. Goodarzi, S. Ababzadeh, M. Sagharjoghi Farahani, S. Mohandesnezhad, N. Bahrami, I. Nabipour, and J. Ai, Int. J. Biol. Macromol., 153, 1241 (2020).
C. Bi, X. Li, Q. Xin, W. Han, C. Shi, R. Guo, W. Shi, R. Qiao, X. Wang, and J. Zhong, J. Biosci. Bioeng., 128, 234 (2019).
G. Dharunya, N. Duraipandy, R. Lakra, P. S. Korapatti, R. Jayavel, and M. S. Kiran, Biomed. Mater., 11, 045011 (2016).
N. N. Fathima, R. S. Devi, K. B. Rekha, and A. Dhathathreyan, J. Chem. Sci., 121, 509 (2009).
M. E. Plonska-Brzezinska, D. M. Bobrowska, A. Sharma, P. Rodziewicz, M. Tomczyk, J. Czyrko, and K. Brzezinski, RSC Adv., 5, 95443 (2015).
Y. Arima and H. Iwata, Biomaterials, 28, 3074 (2007).
M. Ranjbar-Mohammadi and S. H. Bahrami, Int. J. Biol. Macromol., 84, 448 (2016).
W. Cui, X. Zhu, Y. Yang, X. Li, and Y. Jin, Mater. Sci. Eng. C, 29, 1869 (2009).
Y. Yang, X. Zhu, W. Cui, X. Li, and Y. Jin, Macromol. Mater. Eng., 294, 611 (2009).
Y. E. Bulbul, M. Okur, F. Demirtas-Korkmaz, and N. Dilsiz, Appl. Clay Sci., 186, 105430 (2020).
A. Shababdoust, M. Zandi, M. Ehsani, P. Shokrollahi, and R. Foudazi, Int. J. Pharm., 575, 118947 (2020).
A. Sadeghianmaryan, Z. Yazdanpanah, Y. A. Soltani, H. A. Sardroud, M. H. Nasirtabrizi, and X. Chen, J. Biomater. Sci. Polym. Ed., 31, 169 (2020).
G. I. Fakhrullina, F. S. Akhatova, Y. M. Lvov, and R. F. Fakhrullin, Environ. Sci. Nano, 2, 54 (2015).
Bhawana, R. K. Basniwal, H. S. Buttar, V. K. Jain, and N. Jain, J. Agric. Food Chem., 59, 2056 (2011).
D. Zheng, C. Huang, H. Huang, Y. Zhao, M. R. U. Khan, H. Zhao, and L. Huang, Chem. Biodivers., 17, e2000171 (2020).
M. Saadipour, A. Karkhaneh, and M. Haghbin Nazarpak, Int. J. Polym. Mater. Polym. Biomater., 71, 386 (2022).
Acknowledgments
A. Hernández Rangel gratefully acknowledges the post-doctoral fellowship provided by the CONACyT México.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare no conflict of interest.
Rights and permissions
About this article
Cite this article
San Martín-Martínez, E., Casañas-Pimentel, R., Almaguer-Flores, A. et al. Curcumin-loaded Polycaprolactone/Collagen Composite Fibers as Potential Antibacterial Wound Dressing. Fibers Polym 23, 3002–3011 (2022). https://doi.org/10.1007/s12221-022-4275-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12221-022-4275-0