Abstract
To find a more sensitive and earlier diagnostic marker for hepatocellular carcinoma, methylation-profils of CpG islands in the promoter of eleven genes in hepatocellular carcinoma(HCC) carcinoma and pericarcinoma tissues from ten patients were examined by using methylation-specific PCR (MSP) method. MSP was used to detect the methylation of p16, p53, Secreted frizzled-related protein 1 (SFRP1) and SFRP2 promoters. Meanwhile, plasma alpha-fetoprotein (AFP) levels in 53 patients were also determined. The results showed that HCC was closely correlated to methylation in promoter of tumor-suppressing gene p16, p53, SFRP1 and SFRP2. The results suggest that methylation of SFRP2 promoter is possible a better marker for diagnosis of HCC than plasma Alpha-fetoprotein (AFP) levels. The false negative of SFRP1 and SFRP2 are perfectly complementary. If SFRP1 and SFRP2 were both considered as a complementary positive marker at the same time, the accurate rate for diagnosis of HCC is 100%.
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Foundation item: Suported by the Scientific and Technology Bureau of Hubei Province Foundation (2005AA301C26)
Biography: LIU Yanhong(1975–), female, Ph. D. candidate, research direction: diagnose and prevention of chronic liver disease.
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Liu, Y., Wu, J., Hu, X. et al. Diagnosis of hepatocellular carcinoma by using methylation specific-PCR. Wuhan Univ. J. of Nat. Sci. 12, 558–562 (2007). https://doi.org/10.1007/s11859-006-0096-z
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DOI: https://doi.org/10.1007/s11859-006-0096-z