Abstract
Chinese hamster ovary (CHO) cell lines have been widely used to produce recombinant proteins. While the biosynthesis of recombinant proteins is energy-intensive, CHO cells exhibit inefficient metabolism, characterized by rapid conversion of glucose to lactate, possibly leading to lower cell growth and productivity of therapeutic proteins. Therefore, it is important to understand and engineer cellular metabolism to increase recombinant protein production. In this review, cellular energy metabolism of CHO cells with respect to protein synthesis is overviewed. Then, genetic and process engineering approaches to enhance metabolic efficiency are described, resulting in the improvement of cell culture performance. Finally, recent modeling technologies for understanding and predicting cellular metabolic behaviors are reviewed. These efforts will aid to further advance the biomanufacturing of therapeutic proteins.
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This work was supported by INHA University Research Grant.
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Jong Youn Baik obtained B.S. and Ph.D. degree in Biological Science from Korea Advanced Institute of Science and Technology (KAIST), Korea in 2003 and 2009, respectively. He was a post-doctoral research fellow at Rensselaer Polytechnic Institute (RPI; 2009–2010), College of Nanoscale Science and Engineering (CNSE; 2010–2013), and University of Delaware (2013–2016) in USA. He was a research associate and research assistant professor in University of Delaware (2016–2019) and an associate site director, UD site in Advanced Mammalian Biomanufacturing Innovation Center (AMBIC; 2016–2019). He joined Inha University in 2019 and is currently an Assistant Professor in Biological Engineering. He has published more than 20 papers and was awarded the grand prize at Korea Biopharmaceuticals Award in 2021.
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Park, J.U., Han, HJ. & Baik, J.Y. Energy metabolism in Chinese hamster ovary (CHO) cells: Productivity and beyond. Korean J. Chem. Eng. 39, 1097–1106 (2022). https://doi.org/10.1007/s11814-022-1062-y
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DOI: https://doi.org/10.1007/s11814-022-1062-y