Abstract
The precipitation efficiency of paclitaxel from Taxus chinensis was remarkably improved through negative pressure cavitation fractional precipitation. When paclitaxel was precipitated under a negative pressure of −200 mmHg, almost all of the paclitaxel (<97%) could be recovered in a short operation time (1 min). The precipitation rate constant was calculated using the JMAK equation for kinetic analysis. The rate constant in the case of negative pressure (−50 to −200 mmHg) cavitation fractional precipitation increased by 2.147–6.046 times compared to fractional precipitation without negative pressure. The change of activation energy by the negative pressure (−50 to−200 mmHg) was also calculated using the Arrhenius equation. The activation energy changes were −1,767 to −4,161 J/mol, implying that the activation energy could be reduced by introducing negative pressure, resulting in an increased precipitation rate. In addition, the application of negative pressure reduced the size of the precipitate by 3.3 times and increased the diffusion coefficient of paclitaxel by 4.4 times.
Article PDF
Avoid common mistakes on your manuscript.
References
L. Zhu and L. Chen, Cell. Mol. Biol. Lett., 24, 40 (2019).
J. M. Park and J. H. Kim, Korean Chem. Eng. Res., 59, 106 (2021).
Y. Wei, X. Pu and L. Zhao, Oncol. Rep., 37, 3159 (2017).
Z. Tan, Q. Li, C. Wang, W. Zhou, Y. Yang, H. Wang, Y. Yi and F. Li, Molecules, 22, 1483 (2017).
M. Ghorbani, F. Pourjafar, M. Saffari and Y. Asgari, Meta Gene, 26, 100800 (2020).
T. Sun, Y. Liu, M. Li, H. Yu and H. Piao, Mol. Cell. Probes, 53, 101602 (2020).
B. Modarresi-Darreh, K. Kamali, S. M. Kalantar, H. Dehghanizadeh and B. Aflatoonian, Eurasia J. Biosci., 12, 413 (2018).
M. Shirshekanb, H. Rezadoost, M. Javanbakht and A. R. Ghassempour, Iran. J. Pharm. Res., 16, 1396 (2017).
H. J. Kang and J. H. Kim, Process Biochem., 99, 316 (2020).
H. W. Seo and J. H. Kim, Process Biochem., 87, 238 (2019).
S. H. Pyo, H. B. Park, B. K. Song, B. H. Han and J. H. Kim, Process Biochem., 39, 1985 (2004).
T. J. McPartland, R. A. Patil, M. F. Malone and S. C. Roberts, Biotechnol. Prog., 28, 990 (2012).
J. H. Kim, I. S. Kang, H. K. Choi, S. S. Hong and H. S. Lee, Biotechnol. Lett., 22, 1753 (2000).
S. B. Oguzkan, B. Karagul, A. Uzun, O. O. Guler and H. I. Ugras, Int. J. Pharmacol., 14, 76 (2018).
S. I. Jeon, S. Y. Mun and J. H. Kim, Process Biochem., 41, 276 (2006).
K. Y. Jeon and J. H. Kim, Process Biochem., 44, 736 (2009).
J. Y. Lee and J. H. Kim, Process Biochem., 47, 2388 (2012).
C. G. Lee and J. H. Kim, Process Biochem., 49, 1370 (2014).
B. S. Schueller and R. T. Yang, Ind. Eng. Chem. Res., 40, 4912 (2001).
H. J. Kang and J. H. Kim, Biotechnol. Bioprocess Eng., 24, 513 (2019).
S. R. Oh and J. H. Kim, Korean J. Chem. Eng., 38, 480 (2021).
S. V. Dalvi and R. N. Dave, Int. J. Pharm., 387, 172 (2010).
C. G. Lee and J. H. Kim, Process Biochem., 59, 216 (2017).
S. V. Dalvi and M. D. Yadav, Ultrason. Sonochem., 24, 114 (2015).
H. J. Kang and M. S. Thesis, Kongju National University, Cheonan, Korea (2021).
P. Khadka, J. Ro, H. Kim, I. Kim, J. T. Kim, H. Kim, J. M. Cho, G. Yun and J. Lee, Asian J. Pharm. Sci., 9, 304 (2014).
D. Ma, J. S. Marshall and J. Wu, J. Acoust. Soc. Am., 114, 3496 (2018).
Z. Guo, A. G. Jones and N. Li, Chem. Eng. Sci., 61, 1617 (2008).
L. Wolloch and J. Kost, J. Control. Release, 148, 204 (2010).
Acknowledgements
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Government of Korea (MSIT) (Grant Number: 2021R1A2C1003186).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Min, HS., Kim, JH. Negative pressure cavitation fractional precipitation for the purification of paclitaxel from Taxus chinensis. Korean J. Chem. Eng. 39, 58–62 (2022). https://doi.org/10.1007/s11814-021-0959-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-021-0959-1