Abstract
Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an engineered tyrosinase (mTyr-CNK) with high catalytic activity for tyrosine/DOPA-tethered polymeric biomaterials throughout a broad pH range. A dual-barrel syringe with one part containing chitosan/mTyr-CNK solution and the other containing gelatin solution with/without nanohydroxyapatite was successfully used to form homogeneous hydrogels at room temperature followed by 37 °C to simulate an in situ injection approach. The obtained hydrogels exhibited an average pore size greater than 150 µm and high swelling ratios with similar mechanical properties to other chemically crosslinked chitosan/gelatin hydrogels. The in vitro degradation properties and cellular viability suggested that the hydrogels could be used as biodegradable and biocompatible scaffolds for biomedical applications, such as space filling biomaterials and delivery vehicles for bioactive molecules and cells. These results demonstrated that mTyr-CNK-mediated hydrogels have remarkable promise as an injectable scaffold biomaterial.
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Acknowledgements
This work was supported by the Marine Biomaterials Research Center grant from Marine Biotechnology Program of the Korea Institute of Marine Science & Technology Promotion funded by the Ministry of Oceans and Fisheries, Korea and by the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (NRF- 2020R1I1A3072957).
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Lim, S., Jeong, D., Ki, MR. et al. Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel. Korean J. Chem. Eng. 38, 98–103 (2021). https://doi.org/10.1007/s11814-020-0672-5
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DOI: https://doi.org/10.1007/s11814-020-0672-5