Abstract
The facile and rapid monitoring of the cellular response to environmental stresses is crucial for understanding the effects of environmental toxicity in living organisms. The overall cell growth can be examined to find a simple monitoring system. Green fluorescent protein (GFP) is advantageous when used as a reporter protein of the cellular stress responses in Escherichia coli because it allows the non-invasive monitoring of GFP in vivo without affecting the cell metabolism. We compared the environmental toxicities of chemical pollutants using GFP expressing E. coli for easy monitoring by incubation in various concentrations of harmful chemicals (ethanol, phenol, para-formaldehyde, paraben, and triclosan). The results showed that all the chemical pollutants act on cell growth and the cell metabolism according to the measured cell density and fluorescent intensity of GFP. In addition, from comparative analysis for quantification, the concentration of unknown ethanol toxicity, which was not determined at that concentration, could be deduced. In conclusion, the degree of toxicity for each chemical pollutant could be estimated or evaluated. This system will be useful for monitoring the toxicity of chemical pollutants in a non-invasive monitoring system.
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References
A. Blom, W. Harder and A. Matin, Appl. Environ. Microb., 58, 331 (1992).
A.D. Grossman, W. E. Taylor, Z. F. Burton, R.R. Burgess and C.A. Gross, J. Mol. Biol., 186, 357 (1985).
F. C. Neidhardt and R.A. VanBogelen, Biochem. Biophys. Res. Commun., 100, 894 (1981).
T. K. Van Dyk, W.R. Majarian, K. B. Konstantinov, R. M. Young, P. S. Dhurjati and R. A. LaRossa, Appl. Environ. Microb., 60, 1414 (1994).
T. Yura, H. Nagai and H. Mori, Annu. Rev. Microbiol., 47, 321 (1993).
J. P. Hendrick and F.-U. Hartl, Annu. Rev. Biochem., 62, 349 (1993).
L. Sedger and J. Ruby, J. Virol., 68, 4685 (1994).
S. Birnbaum and J. E. Bailey, Biotechnol. Bioeng., 37, 736 (1991).
S. A. Goff and A. L. Goldberg, Cell, 41, 587 (1985).
S.W. Harcum and W.E. Bentley, Biotechnol. Bioeng., 42, 675 (1993).
A. Matin, Mol. Microbiol., 5, 3 (1991).
A.C. St John and A. L. Goldberg, J. Bacteriol., 143, 1223 (1980).
D. E. Jenkins, E.A. Auger and A. Matin, J. Bacteriol., 173, 1992 (1991).
T.K. Van Dyk, T.R. Reed, A.C. Vollmer and R. A. LaRossa, J. Bacteriol., 177, 6001 (1995).
R.A. VanBogelen, P.M. Kelley and F.C. Neidhardt, J. Bacteriol., 169, 26 (1987).
S.R. Ahmed, K. Koh, E.Y. Park, J. Lee, Korean J. Chem. Eng., 30, 1825 (2013).
A. Crameri, E.A. Whitehorn, E. Tate and W.P.C. Stemmer, Nat. Biotechnol., 14, 315 (1996).
M. Chalfie, Y. Tu, G. Euskirchen, W. Ward and D. Prasher, Science, 263, 802 (1994).
H. J. Cha, R. Srivastava, V. M. Vakharia, G. Rao and W. E. Bentley, Appl. Environ. Microb., 65, 409 (1999).
H. J. Yoo, J. H. Seo, D. G. Kang and H. J. Cha, Korean J. Chem. Eng., 24, 99 (2007).
S. Ravikumar, I. Ganesh, M. K. Maruthamuthu, S. H. Hong, Korean J. Chem. Eng., 32, 2073 (2015).
S. P. Rupani, M. B. Gu, K. B. Konstantinov, P. S. Dhurjati, T. K. Van Dyk and R. A. LaRossa, Biotechnol. Prog., 12, 387 (1996).
K.M. Dombek and L.O. Ingram, J. Bacteriol., 157, 233 (1984).
G. McDonnell and A.D. Russell, Clin. Microbiol. Rev., 12, 147 (1999).
W. F. Bergfeld, D.V. Belsito, J. G. Marks Jr. and F. A. Andersen, J. Am. Acad. Dermatol., 52, 125 (2005).
R. Golden, J. Gandy and G. Vollmer, Crit. Rev. Toxicol., 35, 435 (2005).
P.W. Harvey and P. Darbre, J. Appl. Toxicol., 24, 167 (2004).
M.G. Soni, I.G. Carabin and G.A. Burdock, Food Chem. Toxicol., 43, 985 (2005).
H.N. Bhargava and P.A. Leonard, Am. J. Infect. Control, 24, 209 (1996).
R. J. Heath, J.R. Rubin, D.R. Holland, E. Zhang, M.E. Snow and C.O. Rock, J. Biol. Chem., 274, 11110 (1999).
E.G.M. Power, Prog. Med. Chem., 34, 149 (1995).
A.D. Russell and D. Hopwood, Prog. Med. Chem., 13, 271 (1976).
R. J.V. Pulvertaft and G.D. Lumb, J. Hyg., 46, 62 (1948).
R. B. Srivastava and R. E. Thompson, Br. J. Exp. Pathol., 47, 315 (1966).
R. B. Srivastava and R. E.M. Thompson, Nature, 206, 216 (1965).
A. Hernández, F. M. Ruiz, A. Romero and J. L. Martínez, Plos Pathog., 7, e1002103 (2011).
J.-Y. Maillard, J. Appl. Microbiol., 92(Suppl), 16S (2002).
S. Saleh, R. N. S. Haddadin, S. Baillie and P. J. Collier, Lett. Appl. Microbiol., 52, 87 (2010).
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Song, Y.H., Kim, C.S. & Seo, J.H. Noninvasive monitoring of environmental toxicity through green fluorescent protein expressing Escherichia coli . Korean J. Chem. Eng. 33, 1331–1336 (2016). https://doi.org/10.1007/s11814-015-0253-1
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DOI: https://doi.org/10.1007/s11814-015-0253-1