Abstract
Amine functionalized cobalt ferrite (AF-CoFe2O4) magnetic nanoparticles (MNPs) were used for immobilization of cellulase enzyme via 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDS) and N-hydroxy-succinimide (NHS) coupling reaction. The structural, morphological and magnetic properties of AF-CoFe2O4 were determined. TEM micrograph revealed a mean diameter of ∼8 nm and showed that the AF-CoFe2O4 remain distinct with no significant change in size after binding with cellulase. Fourier transform infrared (FT-IR) spectroscopy confirmed the binding of cellulase to AF-CoFe2O4. The properties of immobilized cellulase were investigated by optimizing binding efficiency, pH, temperature and reusability. The results showed that the immobilized cellulase has higher thermal stability than free cellulase, which might be due to covalent interaction between cellulase and AF-CoFe2O4 surface. The immobilized cellulase also showed good reusability after recovery. Therefore, AF-CoFe2O4 MNPs can be considered as promising candidate for enzyme immobilization.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
K. E. Sapsford, W. R. Algar, L. Berti, K. B. Gemmill, B. J. Casey, E. Oh, M. H. Stewart and I. L. Medintz, Chem. Rev., 113, 1904 (2013).
E. Amstad, M. Textor and E. Reimhult, Nanoscale, 3, 2819 (2011).
K. Hola, Z. Markova, G. Zoppellaro, J. Tucek and R. Zboril, Biotechnol. Adv. (2015), DOI:10. 1016/j. biotechadv. 2015. 02. 003.
C. Xu and S. Sun, Adv. Drug Deliv. Rev., 65, 732 (2013).
J. Xu, J. Sun, Y. Wang, J. Sheng, F. Wang and M. Sun, Molecules, 19, 11465 (2014).
R. A. Bohara and S. H. Pawar, Appl. Biochem. Biotechnol., 176(4), 1044 (2015).
R. A. Bohara, N. D. Thorat, A. K. Chaurasia and S. H. Pawar, RSC Adv., 5, 47225 (2015).
S. A. Ansari and Q. Husain, Biotechnol. Adv., 30, 512 (2012).
D. T. Nguyen and K.-S. Kim, Korean J. Chem. Eng., 31, 1289 (2014).
N. T. K. Thanh and L. A. W. Green, Nano Today, 5, 213 (2010).
S.-N. Sun, C. Wei, Z.-Z. Zhu, Y.-L. Hou, S. S. Venkatraman and Z.-C. Xu, Chinese Phys. B, 23, 037503 (2014).
S. Mohapatra, S. R. Rout, S. Maiti, T. K. Maiti and A. B. Panda, J. Mater. Chem., 21, 9185 (2011).
C. Mateo, J. M. Palomo, G. Fernandez-Lorente, J. M. Guisan and R. Fernandez-Lafuente, Enzyme Microb. Technol., 40, 1451 (2007).
C. G. C. M. Netto, H. E. Toma and L. H. Andrade, J. Mol. Catal. B Enzym., 85-86, 71 (2013).
L. Zang, J. Qiu, X. Wu, W. Zhang, E. Sakai and Y. Wei, J. Magn. Magn. Mater., 375, 117 (2015).
A. Sassolas, L. J. Blum and B. D. Leca-Bouvier, Biotechnol. Adv., 30, 489 (2012).
J. Jordan, C. S. S. R. Kumar and C. Theegala, J. Mol. Catal. B Enzym., 68, 139 (2011).
K. Khoshnevisan, A. K. Bordbar, D. Zare, D. Davoodi, M. Noruzi, M. Barkhi and M. Tabatabaei, Chem. Eng. J., 171, 669 (2011).
K. M. Ho, X. Mao, L. Gu and P. Li, Langmuir, 24, 11036 (2008).
D. Brady and J. Jordaan, Biotechnol. Lett., 31, 1639 (2009).
S. Amiri and H. Shokrollahi, Mater. Sci. Eng. C, 33, 1 (2013).
R. A. Bohara, N. D. Thorat, H. M. Yadav and S. H. Pawar, New J. Chem., 38, 2979 (2014).
S. Mohapatra, S. R. Rout and A. B. Panda, Colloids Surfaces A Physicochem. Eng. Asp., 384, 453 (2011).
V. Swarnalatha, R. Aluri Esther and R. Dhamodharan, J. Mol. Catal. B Enzym., 96, 6 (2013).
R. A. Bohara, H. M. Yadav, N. D. Thorat, S. S. Mali, C. K. Hong, S. G. Nanaware and S. H. Pawar, J. Magn. Magn. Mater., 378, 397 (2015).
S. Gyergyek, D. Makovec, A. Kodre, I. Arcon, M. Jagodic and M. Drofenik, J. Nanoparticle Res., 12, 1263 (2010).
N. D. Thorat, S. V. Otari, R. M. Patil, R. A. Bohara, H. M. Yadav, V. B. Koli, A. K. Chaurasia and R. S. Ningthoujam, Dalton Trans., 43, 17343 (2014).
S. Kumar, A. K. Jana, M. Maiti and I. Dhamija, J. Nanoparticle Res., 16, 2233 (2014).
A. A. Bogdanov, A. L. Klibanov and V. P. Torchilin, FEBS Lett., 231, 381 (1988).
N. M. Mubarak, J. R. Wong, K. W. Tan, J. N. Sahu, E. C. Abdullah, N. S. Jayakumar and P. Ganesan, J. Mol. Catal. B Enzym., 107, 124 (2014).
W. Zhang, J. Qiu, L. Zang, E. Sakai and H. Feng, Nano, 10, 1550013 (2015).
R. E. Abraham, M. L. Verma, C. J. Barrow and M. Puri, Biotechnology for Biofuels, 7, 90 (2014).
W. Zhang, J. Qiu, H. Feng, L. Zang and E. Sakai, J. Magn. Magn. Mater., 375, 117 (2015).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bohara, R.A., Thorat, N.D. & Pawar, S.H. Immobilization of cellulase on functionalized cobalt ferrite nanoparticles. Korean J. Chem. Eng. 33, 216–222 (2016). https://doi.org/10.1007/s11814-015-0120-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-015-0120-0