Abstract
The objective of this study was to enhance the lipid productivity in microalga Tetraselmis sp. through the salinity variation during cultivation. When marine alga Tetraselmis sp. was cultivated in a wide range of salinities, 0 through 70 practical salinity unit (PSU), enriched with F/2 medium, relatively low salinities below 35 PSU resulted in higher growth rates and lipid productivities under both N-deficient and -sufficient conditions, as compared to high salinities above 45 PSU. Nitrogen limitation did not stimulate lipid production in this species. Although high salinity increased lipid content, overall lipid productivities were lowered than those under low salinity conditions due to the decreased biomass production. When the salinity shifted from 35 to 22 PSU during cultivation, total lipid content increased from 20 to 26 (w/w) % within four days, and no significant change of fatty acids composition was observed.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
J. K. Kim, B.-H. Um and T. H. Kim, Korean J. Chem. Eng., 29, 209 (2012).
M. J. Asadollahzadeh, M. Ardjmand, A. A. Seafkordi and S. M. Heydarian, Korean J. Chem. Eng., 31, 1425 (2014).
G. Kim, W. Choi and K. Lee, Biochem. Eng. J., 78, 18 (2013).
H. M. Amaro, A. C. Guedes and F. X. Malcata, Appl. Energy, 88, 3402 (2011).
G. Kim, G. Mujtaba, R. Muhamad and K. Lee, Appl. Chem. Eng., 25, 553 (2014).
E. Suali and R. Sarbatly, Renew. Sust. Energ. Rev., 16, 4316 (2012).
G. Mujtaba, W. Choi, C. Lee and K. Lee, Bioresour. Technol., 123, 279 (2012).
L. Rodolfi, G. C. Zittelli, N. Bassi, G. Padovani, N. Biondi, G. Bonini and M. R. Tredici, Biotechnol. Bioeng., 102, 100 (2009).
M. Chen, H. Tang, H. Ma, T. C. Holland, K. Y. Simon Ng and S. O. Salley, Bioresour. Technol., 102, 1649 (2011).
S. V. Mohan and M. P. Devi, Bioresour. Technol., 165, 288 (2014).
M. L. Bartley, W. J. Boeing, A. A. Corcoran, F. O. Holguin and T. Schaub, Biomass Bioenergy, 54, 83 (2013).
P. Kaewkannetra, P. Enmak and T. Chiu, Biotechnol. Bioprocess Eng., 17, 591 (2012).
Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, M. Seibert and A. Darzins, Plant J., 52, 621 (2008).
S. H. Ho, C. Y. Chen and J. S. Chang, Bioresour. Technol., 113, 244 (2012).
G. Breuer, P. P. Lamers, D. E. Martens, R. B. Draaisma and R. H. Wijffels, Bioresour. Technol., 124, 217 (2012).
R. Huerlimann, R. Nys and K. Heimann, Biotechnol. Bioeng., 107, 245 (2010).
C. G. Lee and D. H. Sung, Korean Patent (Pending) 10-2012-0085961 (2012).
C. Yao, J. Ai, X. Cao, S. Xue and W. Zhang, Bioresour. Technol., 118, 438 (2012).
P. Bondioli, L. D. Bella, G. Rivolta, G. C. Zittelli, N. Bassi, L. Rodolfi, D. Casini, M. Prussi, D. Chiaramonti and M. R. Tredici, Bioresour. Technol., 114, 567 (2012).
C. J. Zhu and Y. K. Lee, J. Appl. Phycol., 9, 189 (1997).
E. G. Bligh and W. J. Dyer, Can. J. Biochem. Physiol., 37, 911 (1959).
S. V. Wychen and L. M. L. Laurens, Determination of total lipids as fatty acid methyl esters (FAME) by in situ transesterification, Laboratory Analytical Procedure, NREL, USA (2013).
M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, Anal. Chem., 28, 350 (1956).
Y. Jiang and F. Chen, J. Ind. Microbiol. Biotechnol., 23, 508 (1999).
G. O. Kirst, Ann. Rev. Plant Biol., 41, 21 (1990).
G. Q. Chen, Y. Jiang and F. Chen, J. Phycol., 44, 1309 (2008).
H. W. Yen, I. C. Hu, C. Y. Chen and J. S. Chang, Design of photobioreactors for algal cultivation, In: A. Pandey, D. J. Lee, Y. Chisti and C. R. Soccol (Eds.), Biofuels from Algae, Elsevier, Burlington (2014).
M. Takagi, Karseno and T. Yoshida, J. Biosci. Bioeng., 101, 223 (2006).
P. C. Gorain, S. K. Bagchi and N. Mallick, Environ. Technol., 34, 1887 (2013).
L. Zhu, X. Zhang, L. Ji, X. Song and C. Kuang, Process Biochem., 42, 210 (2007).
M. Azachi, A. Sadka, M. Fisher, P. Goldshlad, I. Gokhman and A. Zamir, Plant Physiol., 129, 1320 (2002).
E. G. Linda, M. G. James and W. W. Lee, Algae. 2nd Ed., Pearson (2009).
A. J. Klok, P. P. Lamers, D. E. Martens, R. B. Draaisma and R. R. Wiffels, Trends Biotechnol., 32, 521 (2014).
W. Q. Xu and J. Beardall, Phytochemistry, 45, 655 (1997).
S. M. Renaud and D. L. Parry, J. Appl. Phycol., 6, 347 (1994).
D. Pal, I. Khozin-Goldberg, Z. Cohen and S. Boussiba, Appl. Microbiol. Biotechnol., 90, 1429 (2011).
M. Xiao, H.-J. Shin and Q. Dong, Korean J. Chem. Eng., 30, 2119 (2013).
H. Tang, N. Abunasser, M. E. D. Garcia, M. Chen, K. Y. Simon Ng and S. O. Sally, Appl. Energy, 88, 3324 (2011).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, G., Lee, CH. & Lee, K. Enhancement of lipid production in marine microalga Tetraselmis sp. through salinity variation. Korean J. Chem. Eng. 33, 230–237 (2016). https://doi.org/10.1007/s11814-015-0089-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11814-015-0089-8