Abstract
The extraction of lipid from Enteromorpha intestinalis was investigated. Among 13 types of organic solvent, the chloroform/methanol (2: 1, v/v) system obtained the highest content. Considering the lipid extraction procedure and extracted amount, the optimal solid/liquid ratio was 1: 50 (w/v). With increasing extraction temperature, the content was enhanced until 45 °C, after which it increased only slightly. Correspondingly, with increasing extraction time, the content linearly increased until 2.5 hr, after which it held steady. From a fatty acid compositional analysis of the lipid extracted from E. intestinalis, the content of palmitic acid and stearic acid was 39.85±1.19% and 14.61±0.80%, respectively. The unsaturated fatty acids (C18:1, C18:2 and C18:3) concentrations, meanwhile, were 0.3-3.2%.
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Jeong, GT., Park, DH. Optimization of lipid extraction from marine green macro-algae as biofuel resources. Korean J. Chem. Eng. 32, 2463–2467 (2015). https://doi.org/10.1007/s11814-015-0083-1
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DOI: https://doi.org/10.1007/s11814-015-0083-1