Abstract
The hydrodynamic characteristic performance of an internal draft tube inverse fluidized bed biofilm reactor was studied for the aerobic biodegradation of phenol (1,200 mg/l) using Pseudomonas fluorescens for various ratios of settled bed volume to reactor working volume (V b /V r ) under batchwise condition with respect to liquid phase. The operating parameters, such as superficial gas velocity, phase hold ups, aspect ratio and bed height, were analyzed for different ratios of (V b /V r ). The effect of biodegradation on synthetic phenolic effluent was determined from the reduction in chemical oxygen demand and phenol removal efficiency. The optimum value of (V b /V r ) m was 0.20 for the optimal superficial gas velocity, U gm =0.220 m/s with the COD reduction efficiency of 98.5% in 48 hours. The biomass and biofilm characteristics of P. fluorescens were determined for optimal hydrodynamic operating parameters by evaluating its biofilm dry density and thickness, bioparticle density, suspended and attached biomass concentration.
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References
L. S. Fan, Gas-liquid-solid fluidization engineering, Butterworths, Boston, USA (1989).
W. Sokol, Int. J. Chem. React. Eng., 8, 1 (2010).
W. Sokol and M. R. Hanafi, Biochem. Eng. J., 3, 185 (1999).
L. Nikolov and D. Karamenew, Can. J. Chem. Eng., 65, 214 (1987).
N. Ulaganathan and K. Krishnaiah, Bioproc. Biosyst. Eng., 15, 159 (1996).
A. Garnier, C. Chavarie, G. Andre and D. Klvana, Chem. Eng. Commun., 98, 31 (1990).
G. Olivieri, A. Marzocchella and P. Salatino, Can. J. Chem. Eng., 88, 574 (2010).
L. S. Fan, K. Muroyama and S. H. Chern, Chem. Eng. J., 24, 143 (1982).
M. Comte, D. Bastoul, G. Hebrard, M. Roustan and V. Lazarova, Chem. Eng. Sci., 52, 3971 (1997).
S.V. Krishna, S. R. Bandaru, D.V. S. Murthy and K. Krishnaiah, China Part., 5, 351 (2007).
V. Sivasubramanian and M. Velan, J. Chem. Eng. Japan, 37, 1436 (2004).
A. Ochieng, T. Ogada, W. Sisenda and P. Wambua, J. Hazard. Mater., 90, 311 (2002).
H. M. Jena, B. K. Sahoo, G. K. Roy and B. C. Meikap, Chem. Eng. J., 145, 50 (2008).
P. Buffiere and R. Moletta, Chem. Eng. Sci., 54, 1233 (1999).
S. J. Han, R. B.H. Tan and K. C. Loh, Trans IChemE, 78, (2000).
T. Renganathan and K. Krishnaiah, Chem. Eng. Sci., 60, 2545 (2005).
L. Gomez, A. Bodalo and E. Gomez, Chem. Eng. J., 127, 47 (2006).
H. S. Choi and M.-S. Shin, Korean J. Chem. Eng., 16(5), 670 (1999).
R. Sowmeyan and C. Swaminathan, Bioresour. Technol., 99, 3877 (2008).
A. A. U. de Souza, H. L. Brandao, I. M. Zamporlini, H.M. Soares and S.M. de A.G.U. de Souza, Res. Conserv. Recycl., 52, 511 (2008).
R. Souza, I.T. L. Bresolin, T. L. Bioni, M. L. Gimenes and B. P. Dias, Braz. J. Chem. Eng., 21, 219 (2004).
S. E. Agarry and B. O. Solomon, Int. J. Environ. Sci. Technol., 5, 223 (2008).
D. Kotresha and G.M. Vidyasagar, Biotechnol. Bioeng., 33, 987 (2007).
APHA-AWA-WPCF1992, Standards methods for the examination of water and waste water, Sixteenth Ed., American Public Health Association, American Water Works Association: Water Pollution Control Federation, Washington, DC, USA (1992).
R. D. Yang and A. E. Humphrey, Biotechnol. Bioeng., 17, 1211 (1975).
S. E. Agarry and B. O. Solomon, Int. J. Environ. Sci. Technol., 5, 223 (2008).
M. Rajasimman and C. Karthikeyan, Front. Chem. Eng. China, 3, 235 (2009).
K. J. F. Rabah and F.M. Dahab, Water Res., 38, 4262 (2004).
L. S. Fan, S. J. Hwang and A. Matsuura, Chem. Eng. Sci., 39, 1677 (1984).
T. Renganathan and K. Krishnaiyah, Can. J. Chem. Eng., 81, 853 (2003).
W. Sokol, A. Ambaw and B. Woldeyes, Chem. Eng. J., 150, 63 (2009).
J.C. Lee and P. S. Buckley, Fluid mechanics and aeration characteristics of fluidized beds, In: P. F. Cooper, B. Atkinson (Ed.), Biological fluidized bed treatment of water and wastewater, Ellis Horwood, Chichester, UK (1981).
W. T. Tang and L. S. Fan, AIChE J., 33, 239 (1987).
G. Olivieri, M. E. Russo, A. Marzocchella and P. Salatino, Biotechnol. Prog., 27, 1599 (2011).
S. D. Kim and Y. Kang, Chem. Eng. Sci., 52, 3639 (1997).
N. Arun, A. A. Razack and V. Sivasubramanian, Chem. Eng. Commun., 200, 1260 (2013).
I. Coelhoso, R. Boatventura and A. Rodriguez, Biotechnol. Bioeng., 40, 625 (1992).
Y. Liu and J.-H. Tay, Water Res., 36, 1653 (2002).
H. Beyenal and A. Tanyolac, Biochem. Eng. J., 1, 53 (1998).
W. K. Kwok, C. Picioreanu, S. L. Ong, M. C.M. van Loosdrecht, W. J. Ng and J. J. Heijnen, Biotechnol. Bioeng., 58, 400 (1998).
K. Fujie, H.Y. Hu, Y. Ikeda and K. Urano, Chem. Eng. Sci., 47, 3745 (1992).
R. C. Chen, J. Reese and L. S. Fan, AIChE J., 40, 1093 (1994).
Y. X. Guo, M. N. Rathor and H.C. Ti, Chem. Eng. J., 67, 205 (1997).
G. A. Hill and C.W. Robinson, Biotechnol. Bioeng., 17, 1599 (1975).
M. Schroeder, C. Muller, C. Posten, W. D. Deckwer, and V. Hecht, Biotechnol. Bioeng., 54, 567 (1997).
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Sabarunisha Begum, S., Radha, K.V. Hydrodynamic behavior of inverse fluidized bed biofilm reactor for phenol biodegradation using Pseudomonas fluorescens . Korean J. Chem. Eng. 31, 436–445 (2014). https://doi.org/10.1007/s11814-013-0260-z
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DOI: https://doi.org/10.1007/s11814-013-0260-z