Abstract
Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPS-induced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.
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Chang, MR., Lee, W.H., Rhee, W.J. et al. Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages. Korean J. Chem. Eng. 30, 1784–1789 (2013). https://doi.org/10.1007/s11814-013-0108-6
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DOI: https://doi.org/10.1007/s11814-013-0108-6