Abstract
Bioactive glass 46S6 and biodegradable therapeutic polymer (Chitosan: CH) have been elaborated to form 46S6-CH composite by freeze-drying process. The kinetics of chemical reactivity and bioactivity at the surface were investigated by using physicochemical techniques, particularly solid-state MAS-NMR. Immortalized cell line used to construct multicellular spheroids was employed as three-dimensional (3D) cell cultures for in vitro studies. Obtained results showed a novel structure of the composite; the chemical treatment (ultrasound, magnetic stirring, freeze drying process and lyophilization) led the bioactive glass particles to be loaded in the chitosan-based materials. 29Si and 31P MAS-NMR results showed the emergence of two new species, Q 3 Si (OH) and Q 4 Si , which are characteristic of the vitreous network dissolution in simulated body fluid (SBF). MAS-NMR also confirmed the formation of amorphous calcium phosphate (ACP) at the surface of the initial 46S6-CH. Three-dimensional (3D) cell cultures highlighted the effect of chitosan, where the cell viability reached up to 78% in 46S6-CH composite and up to 67% in 46S6. The association of (CH) and bioactive glass (BG) matrix promotes a highly significant bioactivity, demonstrating surface bone formation and satisfactory behavior in biological environment.
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References
L. L. Hench, R. J. Splinter, W. C. Allen and T. K. Greenlee, J. Biomed. Mater. Res., 5, 117 (1971).
L. L. Hench, J. Sci. Mater: Mater. Med., 17, 967 (2006).
J. Rich, T. Jaakkola, T. Tirri, T. Narhi, A. Yli-Urpo and C. Seppala, J. Biomater., 23, 2143 (2002).
D. Walsh, T. Furuzono and A. Tanaka, J. Biomater., 22, 1205 (2001).
J. Devin, M. Attawia and C. Laurencin, J. Sci. Biomater. Polymer. Edn., 7, 661 (1996).
I.B. Leonor, E. T. Baran, M. Kawashita, R. L. Reis, T. Kokubo and T. Nakamura, Acta Biomater., 4, 1349 (2008).
W.W. Thein-Han and R. D. K. Misra, Acta Biomater., 5, 1182 (2008).
G. Brandenberg, L.G. Leibrock, R. Shuman and W.G. Malette, Neurosur., 15, 9 (1984).
R. A. Muzzarelli, F. Tanfani, M. Emanuelli, D. P. Pace and E. Chiumzzi, Res. Carbohyd., 126, 225 (1984).
S. Hirano and Y. Yagi, Res. Carbohyd., 8, 103 (1980).
L. Jiang, Y. Li, X. Wang, L. Zhang, J. Wen and M. Gong, Carbohyd. Poly., 74, 680 (2008).
L. Zhao and J. Chang, J. Sci. Mater: Mater. in Med., 15, 625 (2004).
D. Joel Bumgardner et al., US Patent, 0254007 A1 (2007).
P. Mathew, N. S. Binulal, S. Soumya, S. V. Nair, T. Furuike, H. Tamura and R. Jayakumar, Carbohyd. Poly., 79, 281 (2009).
B. D. Boyan, et al., US Patent, 5,492,697 (1996).
E. Dietrich, H. Oudadesse, A. Lucas-Girot and M. Mami, J. Biomed. Mater. Res. A, 88, 1087 (2008).
X.V. Bui, H. Oudadesse, Y. Le Gal, O. Merdrignac-Conanec and G. Cathelineau, Korean J. Chem. Eng., 29(2), 215 (2012).
X. V. Bui, PhD, Elaboration de biomatériaux verres-substances actives (Zoledronate-Chitosan), Order Number 4421, University of Rennes 1, France, 171, 21 November 2011.
T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi and T. Yamamuro, J. Biomed. Mater. Res., 24, 721 (1990).
D. Massiot, F. Fayon, M. Capron, I. King, S. Le Calvé, B. Alonso, J. O. Durand, B. Bujoli, Z. Gan and G. Hoaston, Magn. Reson. Chem., 40, 70 (2002).
M.W.G. Lockyer, D. Holland and R. Dupree, J. Non. Cryst. Solids, 188, 207 (1995).
I. Elgayar, A. E. Aliev, A.R. Boccaccini and R.G. Hill, J. Non. Cryst. Solids, 351, 173 (2005).
A. Angelopoulou, V. Montouillout, D. Massiot and G. Kordas, J. Non. Cryst. Solids, 354, 333 (2008).
Z.R. Hinedi, S. Goldberg, A. C. Chang and J. P. Yesinowski, J. Colloid Interface Sci., 152, 141 (1992).
P. S. Belton, R.K. Harris and P. J. Wilkes, J. Phys. Chem. Solids, 49, 21 (1988).
W. P. Aue, A. H. Roufosse, M. J. Glimcher and R.G. Griffin, Biochem., 23, 6110 (1984).
K. S. K. Lin, Y. H. Tseng, Y. Mou, Y. C. Hsu, C. M. Yang and J. C. C. Chan, Chem. Mater., 17, 4493 (2005).
N.G. Philips, Andy Y. YHLo, I.B. Isabel, G. Ana, A. Daniel, S. Bal tzar, G. Jekabs, V.R. Maria and E. Mattias, J. Phys. Chem. C, 114, 9345 (2010).
A. Diaz, T. Lopez, J. Manjarrez, E. Basaldella, J.M. Martinez-Blanes and J. A. Odriozola, Acta Biomater., 2, 173 (2006).
H. Oudadesse, E. Dietrich, X. V. Bui, Y. Le Gal, P. Pellen and G. Cathelineau, Appl. Surf. Sci., 257, 8587 (2011).
K. J. D. Machenzie and M. E. Smith, Multinuclear solid-state NMR of inorganic materials, Cambridge: Pergamon Mater Series (2002).
R. Oestrike, W. H. Yang, R. J. Kirkpatrick, R. L. Hervig, A. Navrotsky and B. Montez, Geochem. Cosmochim Acta, 51, 2199 (1987).
H. Oudadesse, USTV, October 2011, Rennes, France (2011).
O. H. Anderson and K. H. Karlsson, J. Non. Cryst. Solids, 129, 145 (1991).
N. Alno, F. Jegoux, P. Pellen-Mussi, S. Tricot, H. Oudadesse, G. Cathelineau, G. De Mello and G. J. Biomed, Mater. Res. A, DOI:10. 1002/jbm.a.32818.
E. Dietrich, PhD, Syntheses et etudes physic-chimiques de verres bioactifs denses et poreux, Order Number 3767, University of Rennes 1, France, 205, 17 October 2008.
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Oudadesse, H., Wers, E., Bui, X.V. et al. Chitosan effects on glass matrices evaluated by biomaterial. MAS-NMR and biological investigations. Korean J. Chem. Eng. 30, 1775–1783 (2013). https://doi.org/10.1007/s11814-013-0104-x
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DOI: https://doi.org/10.1007/s11814-013-0104-x