Abstract
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
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Lee, J.H., Kim, S.B., Yoo, H.Y. et al. Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production. Korean J. Chem. Eng. 30, 1335–1338 (2013). https://doi.org/10.1007/s11814-013-0058-z
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DOI: https://doi.org/10.1007/s11814-013-0058-z